Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent manner.

Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent manner. Research Abstract Details 

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Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent manner. Abstract Text:

Xiaofeng Xia,Yingsha Zhang,Caroline R Zieth,Su-Chun Zhang,

Lentiviruses have been increasingly used for genetic modification of human cells including embryonic stem (ES) cells. Using four ubiquitous promoters--cytomegalovirus (CMV), cytomegalovirus immediate-early enhancer/chicken beta-actin hybrid (CAG), phosphoglycerate kinase (PGK), and human elongation factor-1alpha (EF1alpha)--in a lentiviral vector to drive the expression of the enhanced green fluorescent protein (EGFP) gene in human ES cells and mouse ES cells, we determined the extent of EGFP suppression by assessing the percentage of cells that were transduced with the EGFP gene but did not fluoresce green. A much higher level of transgene suppression was observed in human ES cells as compared to mouse ES cells. The suppression was also highly promoter dependent, leading to inactivation of more than 95% of the EGFP genes under the CMV or CAG promoter while only 55% under the PGK promoter. No promoter-dependent suppression was observed in transient transfection of human ES cells. Thus, the common phenomenon of poor transgene expression in human ES cells may be caused mainly by suppression of the transgene right after transduction and integration. Cautions should be taken to choose the optimal promoter when lentiviruses are used for genetic modification of human ES cells.

Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent manner. Publishing Authors By Initials

X Xia,Y Zhang,CR Zieth,SC Zhang,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus integration research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus integration research

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Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent manner. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Stem cells and development

VOLUME: 16

Page Numbers: 167-76

Journal Abbreviation: Stem Cells Dev.

ISSN: 1547-3287

DAY: 3

MONTH: Feb

YEAR: 2007

Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent manner. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101197107

Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent manner. Keywords Mesh Terms:

KEYWORDS: Virus Integration

MESH TERMS: genetics

Chemical & Substance for Abstract: Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent manner. Information

Substance Name: Phosphoglycerate Kinase

Registry Number: EC 2.7.2.3

Grant and Affiliation Information for Transgenes delivered by lentiviral vector are suppressed in human embryonic stem cells in a promoter-dependent manner.

AFFILIATION: WiCell Research Institute, University of Wisconsin-Madison, Madison, WI 53705, USA.

Country: United States

AGENCY: United States NICHD

GRANT: P30 HD03352

ACRONYM: HD

MEDLINETA: Stem Cells Dev

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