Transforming lepidopteran insect cells for improved protein processing.

Transforming lepidopteran insect cells for improved protein processing. Research Abstract Details 

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Transforming lepidopteran insect cells for improved protein processing. Abstract Text:

Robert L Harrison,Donald L Jarvis,

The lepidopteran insect cells used with the baculovirus expression vector system (BEVS) are capable of synthesizing and accurately processing foreign proteins. However, proteins expressed in baculovirus-infected cells often fail to be completely processed, or are not processed in a manner that meets a researcher's needs. This chapter discusses a metabolic engineering approach that addresses this problem. Basically, this approach involves the addition of new or enhancement of existing protein processing functions in established lepidopteran insect cell lines. Methods for engineering these cell lines and assessing their properties as improved hosts for the BEVS are detailed. Examples of lepidopteran insect cell lines engineered for improved protein N-glycosylation and trafficking are described.

Transforming lepidopteran insect cells for improved protein processing. Publishing Authors By Initials

RL Harrison,DL Jarvis,

For similar genetic processes: recombination, genetic: transformation, genetic research abstracts see: genetic processes: recombination, genetic: transformation, genetic research

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Transforming lepidopteran insect cells for improved protein processing. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 388

Page Numbers: 341-56

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 3

MONTH: 12

YEAR: 2007

Transforming lepidopteran insect cells for improved protein processing. Information

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LANGUAGE: eng

NlmUniqueID: 9214969

Transforming lepidopteran insect cells for improved protein processing. Keywords Mesh Terms:

KEYWORDS: Transformation, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Transforming lepidopteran insect cells for improved protein processing. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Transforming lepidopteran insect cells for improved protein processing.

AFFILIATION: USDA/ARS Insect Biocontrol Laboratory, Beltsville, MD, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM49734

ACRONYM: GM

MEDLINETA: Methods Mol Biol

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