Transforming lepidopteran insect cells for continuous recombinant protein expression.

Transforming lepidopteran insect cells for continuous recombinant protein expression. Research Abstract Details 

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Transforming lepidopteran insect cells for continuous recombinant protein expression. Abstract Text:

Robert L Harrison,Donald L Jarvis,

The baculovirus expression vector system is widely used to produce large quantities of recombinant proteins. However, yields of extracellular and membrane-bound proteins obtained with this system often are very low, possibly because of the adverse effects of baculovirus infection on the host insect cell secretory pathway. An alternative approach to producing poorly expressed proteins is to transform insect cells with the gene of interest under the control of promoters that are constitutively active in uninfected cells, thereby making cell lines that continuously express recombinant protein. This chapter provides an overview of the methods and considerations for making stably transformed lepidopteran cells. Techniques for the insertion of genes into continuous expression vectors, transfection of cells, and the selection and isolation of stably transformed Sf-9 clones by either colony formation or end-point dilution are described in detail.

Transforming lepidopteran insect cells for continuous recombinant protein expression. Publishing Authors By Initials

RL Harrison,DL Jarvis,

For similar genetic processes: recombination, genetic: transformation, genetic research abstracts see: genetic processes: recombination, genetic: transformation, genetic research

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Transforming lepidopteran insect cells for continuous recombinant protein expression. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 388

Page Numbers: 299-316

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 3

MONTH: 12

YEAR: 2007

Transforming lepidopteran insect cells for continuous recombinant protein expression. Information

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LANGUAGE: eng

NlmUniqueID: 9214969

Transforming lepidopteran insect cells for continuous recombinant protein expression. Keywords Mesh Terms:

KEYWORDS: Transformation, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Transforming lepidopteran insect cells for continuous recombinant protein expression. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Transforming lepidopteran insect cells for continuous recombinant protein expression.

AFFILIATION: USDA/ARS Insect Biocontrol Laboratory, Beltsville, MD, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM49734

ACRONYM: GM

MEDLINETA: Methods Mol Biol

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