Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction.

Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction. Research Abstract Details 

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Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction. Abstract Text:

Wai-Yee Li,Eunice Y Huang,Marek Dudas,Vesa Kaartinen,David Warburton,Tai-Lan Tuan,

For over two decades, the precise role of transforming growth factor-beta (TGF-beta) isoforms in scarless healing of mammalian fetal skin wounds has generated much interest. Although their exact role remains to be established, it has been suggested that high TGF-beta3 activity may correlate with a scarless phenotype. Previously, we showed that plasminogen activator inhibitor-1 (PAI-1), a known TGF-beta downstream molecule and marker of fibrosis, is also developmentally regulated during fetal skin development. In this study, the relationship between TGF-beta3 and PAI-1 was investigated using embryonic day 14.5 TGF-beta3 knockout (ko) mice. The results showed increased PAI-1 expression in the epidermis and dermis of ko mice, using an ex vivo limb-wounding study. Furthermore, increased PAI-1 expression and activity was seen in embryo extracts and conditioned media of ko dermal fibroblasts. When TGF-beta3 knockout fibroblasts were placed into three-dimensional collagen matrices, they were found to have decreased collagen gel contraction, suggesting altered cell-matrix interaction. These findings provide a further avenue for the interactive role of TGF-beta3 and PAI-1 during fetal scarless repair.

Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction. Publishing Authors By Initials

WY Li,EY Huang,M Dudas,V Kaartinen,D Warburton,TL Tuan,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration: wound healing research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration: wound healing research

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Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Wound repair and regeneration : official publicati

VOLUME: 14

Page Numbers: 516-25

Journal Abbreviation:

ISSN: 1067-1927

DAY: 3

MONTH: 12

YEAR: 2007

Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9310939

Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction. Keywords Mesh Terms:

KEYWORDS: Wound Healing

MESH TERMS: physiology

Chemical & Substance for Abstract: Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction. Information

Substance Name: Collagen

Registry Number: 9007-34-5

Grant and Affiliation Information for Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction.

AFFILIATION: Department of Surgery, The Saban Research Institute of Childrens Hospital, Los Angeles, California 90027, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01GM055081

ACRONYM: GM

MEDLINETA: Wound Repair Regen

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