Transformation of taxol-producing endophytic fungi by restriction enzyme-mediated integration (REMI).

Transformation of taxol-producing endophytic fungi by restriction enzyme-mediated integration (REMI). Research Abstract Details 

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Transformation of taxol-producing endophytic fungi by restriction enzyme-mediated integration (REMI). Abstract Text:

Yechun Wang,Binhui Guo,Zhiqi Miao,Kexuan Tang,Yechun Wang,Binhui Guo,Zhiqi Miao,Kexuan Tang,

The REMI method was used to introduce the plasmid pV2 harboring the hygromycin B phosphotransferase (hph) gene controlled by the Aspergillus nidulans trpC promoter and the trpC terminator into a taxol-producing endophytic fungus BT2. REMI transformation yielded stable transformants capable of continuing to grow on PDA medium containing 125 mug mL(-1) hygromycin B. The transformation efficiency was about 5-6 transformants mug(-1) plasmid DNA. The presence of hph gene in transformants was confirmed by PCR and Southern blot analyses. To the authors' knowledge, this is the first report on the transformation of taxol-producing endophytic fungi by the REMI technique. This study provides an effective approach for improving taxol production of endophytic fungi by the genetic engineering of taxol biosynthetic pathway genes in the future.

Transformation of taxol-producing endophytic fungi by restriction enzyme-mediated integration (REMI). Publishing Authors By Initials

Y Wang,B Guo,Z Miao,K Tang,Y Wang,B Guo,Z Miao,K Tang,

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Transformation of taxol-producing endophytic fungi by restriction enzyme-mediated integration (REMI). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEMS microbiology letters

VOLUME: 273

Page Numbers: 253-9

Journal Abbreviation: FEMS Microbiol. Lett.

ISSN: 0378-1097

DAY: 30

MONTH: 06

YEAR: 2007

Transformation of taxol-producing endophytic fungi by restriction enzyme-mediated integration (REMI). Information

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LANGUAGE: eng

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AFFILIATION: Plant Biotechnology Research Center, School of Agriculture and Biology, School of Life Science and Technology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai, China.

Country: England

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MEDLINETA: FEMS Microbiol Lett

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