Transfer of plastid RNA-editing activity to novel sites suggests a critical role for spacing in editing-site recognition.

Transfer of plastid RNA-editing activity to novel sites suggests a critical role for spacing in editing-site recognition. Research Abstract Details 

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Transfer of plastid RNA-editing activity to novel sites suggests a critical role for spacing in editing-site recognition. Abstract Text:

M Hermann,R Bock,

RNA editing in higher plant plastids alters mRNA sequences by C-to-U conversions at highly specific sites through an unknown mechanism. To elucidate how the cytidine residues to be edited are specifically recognized and distinguished from other cytidines in close proximity, we have changed in vivo the distances of two plastid RNA-editing sites from their essential upstream cis-acting sequence element. Analysis of RNA editing in transgenic chloroplasts revealed that reduction of this distance by 1 nt entirely abolishes RNA editing. Surprisingly, deletions or combinations of deletional and point mutations that shift a heterologous cytidine residue in the same distance from the upstream cis-element as the editing site in the wild type result in transfer of the RNA-editing activity to the heterologous cytidine whereas the wild-type site remains unedited. Our results suggest that the molecular identity of at least some editing sites in the chloroplast genome is defined by their distance from an essential upstream sequence element.

Transfer of plastid RNA-editing activity to novel sites suggests a critical role for spacing in editing-site recognition. Publishing Authors By Initials

M Hermann,R Bock,

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Transfer of plastid RNA-editing activity to novel sites suggests a critical role for spacing in editing-site recognition. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 96

Page Numbers: 4856-61

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 27

MONTH: Apr

YEAR: 1999

Transfer of plastid RNA-editing activity to novel sites suggests a critical role for spacing in editing-site recognition. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

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Grant and Affiliation Information for Transfer of plastid RNA-editing activity to novel sites suggests a critical role for spacing in editing-site recognition.

AFFILIATION: Institut fur Biologie III, Universitat Freiburg, Schanzlestrasse 1, D-79104 Freiburg, Germany.

Country: United States

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MEDLINETA: Proc Natl Acad Sci U S A

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