[Transfer and expression of rh-SCF gene in human hematopoietic stem/progenitor cells]

[Transfer and expression of rh-SCF gene in human hematopoietic stem/progenitor cells] Research Abstract Details 

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[Transfer and expression of rh-SCF gene in human hematopoietic stem/progenitor cells] Abstract Text:

J Wang,Y Zhu,C Lai,S Xue,H Ma,B Shen,

OBJECTIVE: To explore the transfer and expression of recombinant human stem cell factor (rh-SCF) gene in human hematopoietic stem/progenitor cell. METHODS: pLXSN-SCF, a recombinant retrovirus vector of soluble human SCF gene, was constructed by recombinant gene technique. The recombinant plasmid was introduced into virus packaging cells psi2 and PA317 by lipofectin-mediated DNA transfection, and PA317/SCF, the recombinant virus producing cell, was obtained by G418 screening [the virus potency was (2.4-8.5) x 10(5) CFU/ml]. Then, human hematopoietic stem/progenitor cells were transfected with PA317/SCF. The transfer and expression of human SCF gene were assayed by PCR, APAAP immunohistochemical staining and chemoluminance-direct ELISA. RESULTS AND CONCLUSION: retrovirus-mediated rh-SCF gene was successfully transferred and expressed in human hematopoietic stem/progenitor cells.

[Transfer and expression of rh-SCF gene in human hematopoietic stem/progenitor cells] Publishing Authors By Initials

J Wang,Y Zhu,C Lai,S Xue,H Ma,B Shen,

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[Transfer and expression of rh-SCF gene in human hematopoietic stem/progenitor cells] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zaz

VOLUME: 18

Page Numbers: 87-90

Journal Abbreviation: Zhonghua Xue Ye Xue Za Zhi

ISSN: 0253-2727

DAY: 29

MONTH: Feb

YEAR: 1997

[Transfer and expression of rh-SCF gene in human hematopoietic stem/progenitor cells] Information

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LANGUAGE: chi

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AFFILIATION: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.

Country: China

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MEDLINETA: Zhonghua Xue Ye Xue Za Zhi

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