Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells.

Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells. Research Abstract Details 

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Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells. Abstract Text:

M A Picchi,B M Bradt,N R Cooper,R T Ogata,

Previously cloned and sequenced full-length cDNAs for murine C4 and the closely related sex-limited protein (Slp) have been placed into an eucaryotic expression vector. Transfer of these DNA constructs transiently into monkey COS cells or stably into mouse L cells results in the expression and secretion of hemolytically active mouse C4 and mature Slp. We estimate from hemolytic activities that COS and L cells secrete 0.04 and 3%, respectively, of the C4 level found in mouse plasma. Slp expression is consistently only 10-20% that of C4 although the identical expression system is used for both. Our results show subtle but reproducible cell-type-specific differences in C4 maturation; they also indicate that surprisingly large shifts in electrophoretic mobility on SDS-polyacrylamide gels are induced by a small number of amino acid substitutions. The expression of C4 from cDNAs of known sequence provides a starting point for studies of structure/function relationships in C4 employing site-specific mutagenesis and gene transfer.

Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells. Publishing Authors By Initials

MA Picchi,BM Bradt,NR Cooper,RT Ogata,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

PUBMED ID PMID:

MEDLINE DATE:

Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Complement and inflammation

VOLUME: 6

Page Numbers: 442-52

Journal Abbreviation:

ISSN: 1012-8204

DAY: 11

MONTH: 12

YEAR: 1989

Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8903074

Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: genetics

Chemical & Substance for Abstract: Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells. Information

Substance Name: Complement C4

Registry Number: 0

Grant and Affiliation Information for Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells.

AFFILIATION: Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, Calif.

Country: SWITZERLAND

AGENCY: United States NIGMS

GRANT: GM36960

ACRONYM: GM

MEDLINETA: Complement Inflamm

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