Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase.

Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase. Research Abstract Details 

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Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase. Abstract Text:

Y Shirasawa,S Sato,T Osawa,A Hirashima,

To investigate the dominant-negative inhibition of Moloney murine leukemia virus (Mo-MuLV) proliferation by polymerization-defective mutants of reverse transcriptase (RT), we constructed several plasmids harboring the Mo-MuLV RT gene in which the YVDD sequence, one of the conserved sequences in RNA-dependent polymerases, was altered, and then transformed mouse NIH3T3 cells and Escherichia coli with the mutant plasmids. Mouse NIH3T3 cells expressing these mutant RT genes were highly resistant to Mo-MuLV proliferation. The mutant RT expressed in E. coli exhibited no polymerization activity, but it retained its binding activity to the template RNA and inhibited in vitro poly(dG) synthesis occurring with the wild-type RT. These results suggest that the competition for binding of the two types of enzymes to the template is responsible for the resistance to Mo-MuLV proliferation and that the YVDD sequence of Mo-MuLV may be a good target for dominant-negative inhibition of retroviral proliferation.

Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase. Publishing Authors By Initials

Y Shirasawa,S Sato,T Osawa,A Hirashima,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

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Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 119

Page Numbers: 1070-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1996

Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: metabolism

Chemical & Substance for Abstract: Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase. Information

Substance Name: RNA-Directed DNA Polymerase

Registry Number: EC 2.7.7.49

Grant and Affiliation Information for Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase.

AFFILIATION: Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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