Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice.

Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice. Research Abstract Details 

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Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice. Abstract Text:

Chandrabali Ghose,Anuj Kalsy,Alaullah Sheikh,Julianne Rollenhagen,Manohar John,John Young,Sean M Rollins,Firdausi Qadri,Stephen B Calderwood,Ciaran P Kelly,Edward T Ryan,

Clostridium difficile is the leading cause of nosocomial infectious diarrhea. C. difficile produces two toxins (A and B), and systemic and mucosal anti-toxin A antibodies prevent or limit C. difficile-associated diarrhea. To evaluate whether transcutaneous immunization with formalin-treated C. difficile toxin A (CDA) induces systemic and mucosal anti-CDA immune responses, we transcutaneously immunized three cohorts of mice with CDA with or without immunoadjuvantative cholera toxin (CT) on days 0, 14, 28, and 42. Mice transcutaneously immunized with CDA and CT developed prominent anti-CDA and anti-CT immunoglobulin G (IgG) and IgA responses in serum and anti-CDA and anti-CT IgA responses in stool. Sera from immunized mice were able to neutralize C. difficile toxin A activity in an in vitro cell culture assay. CDA itself demonstrated adjuvant activity and enhanced both serum and stool anti-CT IgA responses. Our results suggest that transcutaneous immunization with CDA toxoid may be a feasible immunization strategy against C. difficile, an important cause of morbidity and mortality against which current preventative strategies are failing.

Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice. Publishing Authors By Initials

C Ghose,A Kalsy,A Sheikh,J Rollenhagen,M John,J Young,SM Rollins,F Qadri,SB Calderwood,CP Kelly,ET Ryan,

For similar complex mixtures: biological products: vaccines: toxoids research abstracts see: complex mixtures: biological products: vaccines: toxoids research

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Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Infection and immunity

VOLUME: 75

Page Numbers: 2826-32

Journal Abbreviation: Infect. Immun.

ISSN: 0019-9567

DAY: 19

MONTH: 03

YEAR: 2007

Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 246127

Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice. Keywords Mesh Terms:

KEYWORDS: Toxoids

MESH TERMS: immunology

Chemical & Substance for Abstract: Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice. Information

Substance Name: tcdA protein, Clostridium difficile

Registry Number: 0

Grant and Affiliation Information for Transcutaneous immunization with Clostridium difficile toxoid A induces systemic and mucosal immune responses and toxin A-neutralizing antibodies in mice.

AFFILIATION: Division of Infectious Diseases, Massachusetts General Hospital, Jackson 504, 55 Fruit Street, Boston, MA 02114, USA. etryan@partners.org

Country: United States

AGENCY: United States NIAID

GRANT: U54 AI 057159

ACRONYM: AI

MEDLINETA: Infect Immun

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