Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production.

Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production. Research Abstract Details 

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Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production. Abstract Text:

Nick J P Wierckx,Hendrik Ballerstedt,Jan A M de Bont,Johannes H de Winde,Harald J Ruijssenaars,Jan Wery,Nick J P Wierckx,Hendrik Ballerstedt,Jan A M de Bont,Johannes H de Winde,Harald J Ruijssenaars,Jan Wery,

The unknown genetic basis for improved phenol production by a recombinant Pseudomonas putida S12 derivative bearing the tpl (tyrosine-phenol lyase) gene was investigated via comparative transcriptomics, nucleotide sequence analysis, and targeted gene disruption. We show upregulation of tyrosine biosynthetic genes and possibly decreased biosynthesis of tryptophan caused by a mutation in the trpE gene as the genetic basis for the enhanced phenol production. In addition, several genes in degradation routes connected to the tyrosine biosynthetic pathway were upregulated. This either may be a side effect that negatively affects phenol production or may point to intracellular accumulation of tyrosine or its intermediates. A number of genes identified by the transcriptome analysis were selected for targeted disruption in P. putida S12TPL3. Physiological and biochemical examination of P. putida S12TPL3 and these mutants led to the conclusion that the metabolic flux toward tyrosine in P. putida S12TPL3 was improved to such an extent that the heterologous tyrosine-phenol lyase enzyme had become the rate-limiting step in phenol biosynthesis.

Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production. Publishing Authors By Initials

NJ Wierckx,H Ballerstedt,JA de Bont,JH de Winde,HJ Ruijssenaars,J Wery,NJ Wierckx,H Ballerstedt,JA de Bont,JH de Winde,HJ Ruijssenaars,J Wery,

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Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of bacteriology

VOLUME: 190

Page Numbers: 2822-30

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ISSN: 1098-5530

DAY: 9

MONTH: 11

YEAR: 2007

Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production. Information

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LANGUAGE: eng

NlmUniqueID: 2985120

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Grant and Affiliation Information for Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production.

AFFILIATION: TNO Quality of Life, P.O. Box 5057, 2600 GB, Delft, The Netherlands. nick.wierckx@tno.nl.

Country: United States

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MEDLINETA: J Bacteriol

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