Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes.

Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes. Abstract Text:

Dominique A Glauser,Thierry Brun,Benoit R Gauthier,Werner Schlegel,

BACKGROUND: Physiological long term adaptation of pancreatic beta cells is driven by stimuli such as glucose and incretin hormones acting via cAMP (e.g. GLP-1) and involves regulated gene expression. Several rapidly inducible immediate-early genes (IEGs) have been identified in beta cells. Many of these IEGs code for transcription factors and have the potential to control the transcription of downstream target genes likely involved in long term cellular adaptation. The identity of these target genes has not been determined, and the sequence of events occurring during beta cell adaptation is still unclear. RESULTS: We have developed a microarray-based strategy for the systematic search of targets. In Min6 insulin-secreting cells, we identified 592 targets and 1278 IEGs responding to a co-stimulation with glucose and cAMP. Both IEGs and targets were involved in a large panel of functions, including those important to beta cell physiology (metabolism, secretion). Nearly 200 IEGs were involved in signaling and transcriptional regulation. To find specific examples of the regulatory link between IEGs and targets, target promoter sequences were analyzed in silico. Statistically significant over-representation of AP-1 response elements notably suggested an important role for this transcription factor, which was experimentally verified. Indeed, cell stimulation altered expression of IEG-encoded components of the AP-1 complex, activating AP-1-dependent transcription. Loss and gain-of-function experiments furthermore allowed to validate a new AP-1 regulated gene (sulfiredoxin) among the targets. AP-1 and sulfiredoxin are sequentially induced also in primary cells from rat islets of Langerhans. CONCLUSION: By identifying IEGs and their downstream targets, this study brings a comprehensive description of the transcriptional response occurring after beta cell stimulation, as well as new mechanistic insights concerning the AP-1 transcription factor.

Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes. Publishing Authors By Initials

DA Glauser,T Brun,BR Gauthier,W Schlegel,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC molecular biology

VOLUME: 8

Page Numbers: 54

Journal Abbreviation: BMC Mol. Biol.

ISSN: 1471-2199

DAY: 22

MONTH: 06

YEAR: 2007

Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100966983

Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes.

AFFILIATION: Fondation pour Recherches Médicales, University of Geneva, Geneva, Switzerland. dominique.glauser@medecine.unige.ch <dominique.glauser@medecine.unige.ch>

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: BMC Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes Related Publications

• A 35-year-old woman with an intrasellar and suprasellar lesion.
• An epidermotropic canine papillomavirus with malignant potential contains an E5 gene and establishes a unique genus.
• Antibiotic-containing collagen for the treatment of bone defects.
• Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans.
• Comparative study on interactive computer-aided-learning and computer-aided-testing in patient-based dental training in maxillofacial surgery.
• Effects of the protein environment on the structure and energetics of active sites of metalloenzymes. ONIOM study of methane monooxygenase and ribonucleotide reductase.
• Fluctuations of serum choline in women.
• Hair loss in women.
• High-grade B-cell NHL of the brachial plexus followed by infiltration of the spinal cord.
• Myc and human papillomavirus type 16 E7 genes cooperate to immortalize human keratinocytes.
• Primary central nervous system lymphoma.
• Prognostic significance of molecular biological and immunohistological parameters in gastrointestinal carcinomas.
• Reliability and construct validity of the Automated Neuropsychological Assessment Metrics (ANAM) mood scale.
• Seasonal variations in the choline content of human serum.
• Sequential actions of ERK1/2 on the AP-1 transcription factor allow temporal integration of metabolic signals in pancreatic beta cells.
• The mitochondrial DNA of Xenoturbella bocki: genomic architecture and phylogenetic analysis.
• Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes.
• [A case report contribution to tumors impeding labor.]
• [COMBINED INDUCTION OF LABOR.]
• [Concept: occupational medicine 2000]
• [Copper- and coeruloplasmin level of umbilical cord blood in relation to fetal age]
• [Enzymatic studies of amniotic fluid in Rh-incompatibility]
• [Frequency of malformations at the University Women's Hospital Leipzig from 1941 to 1965. I. Frequency of malformations with special reference to gestosis]
• [Frequency of malformations at the University Women's Hospital Leipzig. II. Frequency of malformations with reference to various anamnestic data]
• [In Process Citation]
• [Not Available.]
• [Prematurity and placental insufficiency]
• [Results and prognostic significance of kidney clearance in late toxemias of pregnancy.]
• [The elderly citizen and the right to work]
• [The influence of the obstetrician in increasing the frequency of lactation with special regard to the puerperium]