Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication.

Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication. Research Abstract Details 

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Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication. Abstract Text:

Cyprian Rossetto,Yang Gao,Irena Yamboliev,Iva ,Gregory Pari,Cyprian Rossetto,Yang Gao,Irena Yamboliev,Iva ,Gregory Pari,Cyprian Rossetto,Yang Gao,Irena Yamboliev,Iva ,Gregory Pari,

Kaposi's sarcoma-associated herpesvirus origin-dependent DNA replication requires the core replication proteins plus K-Rta and K-bZIP. To determine which K-bZIP protein domains contribute to oriLyt-dependent DNA replication and facilitate suppression of K-Rta-mediated transcriptional activation, we generated a series of deletion constructs and site-directed mutations within the K-bZIP ORF. Mutation of key leucine residues within the putative leucine zipper (LZ) motif eliminated the ability of the protein to homodimerize and complement oriLyt-dependent DNA replication. Deletion of the basic amino acid region (BR) or LZ domain did not affect the ability of K-bZIP to bind to K-Rta indicating that either region contributes to heterodimerization with K-Rta. However, deletions or mutations introduced into both the LZ and BR resulted in elimination of the suppressive activity of K-bZIP even in the presence of a K-bZIP-K-Rta interaction. Interestingly, mutants that lacked the ability to suppress K-Rta transactivation were still capable of complementing oriLyt-dependent DNA replication, indicating that this activity does not contribute to the DNA synthesis-related activity of K-bZIP.

Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication. Publishing Authors By Initials

C Rossetto,Y Gao,I Yamboliev,I ,G Pari,C Rossetto,Y Gao,I Yamboliev,I ,G Pari,C Rossetto,Y Gao,I Yamboliev,I ,G Pari,

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Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Virology

VOLUME: 369

Page Numbers: 340-50

Journal Abbreviation: Virology

ISSN: 0042-6822

DAY: 21

MONTH: 09

YEAR: 2007

Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication. Information

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LANGUAGE: eng

NlmUniqueID: 110674

Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication. Keywords Mesh Terms:

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Chemical & Substance for Abstract: Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication. Information

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Grant and Affiliation Information for Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication.

AFFILIATION: University of Nevada, Reno School of Medicine, Department of Microbiology and Immunology and the Cell and Molecular Biology Graduate Program, Howard Bldg. 210, Reno, NV 89557, USA.

Country: United States

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MEDLINETA: Virology

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