Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene.

Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene. Abstract Text:

Yuly F Fuentes Medel,Paul D Gardner,

The genes encoding the nicotinic acetylcholine receptor alpha3, alpha5, and beta4 subunits are genomically clustered. These genes are co-expressed in a variety of cells in the peripheral and central nervous systems. Their gene products assemble in a number of stoichiometries to generate several nicotinic receptor subtypes that have distinct pharmacological and physiological properties. Signaling through these receptors is critical for a variety of fundamental biological processes. Despite their importance, the transcriptional mechanisms underlying their coordinated expression remain to be completely elucidated. By using a bioinformatics approach, we identified a highly conserved intronic sequence within the fifth intron of the alpha3 subunit gene. Reporter gene analysis demonstrated that this sequence, termed "alpha3 intron 5," inhibits the transcriptional activities of the alpha3 and beta4 subunit gene promoters. This repressive activity is position- and orientation-independent. Importantly, repression occurs in a cell type-specific manner, being present in cells that do not express the receptor genes or expresses them at very low levels. Electrophoretic mobility shift assays demonstrate that nuclear proteins specifically interact with alpha3 intron 5 at two distinct sites. We propose that this intronic repressor element is important for the restricted expression patterns of the nicotinic receptor alpha3 and beta4 subunit genes.

Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene. Publishing Authors By Initials

YF Medel,PD Gardner,

For similar genetic processes: gene expression regulation: trans-activation (genetics) research abstracts see: genetic processes: gene expression regulation: trans-activation (genetics) research

PUBMED ID PMID:

MEDLINE DATE:

Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 19062-70

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 15

MONTH: 05

YEAR: 2007

Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene. Keywords Mesh Terms:

KEYWORDS: Trans-Activation (Genetics)

MESH TERMS: physiology

Chemical & Substance for Abstract: Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene. Information

Substance Name: nicotinic receptor subunit alpha3

Registry Number: 0

Grant and Affiliation Information for Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene.

AFFILIATION: Brudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School, Worcester, Massachusetts 01604, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS30243

ACRONYM: NS

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene Related Publications

• A witnessed short fall mimicking presumed shaken baby syndrome (inflicted childhood neurotrauma).
• CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges.
• Cardiopulmonary resuscitation in pregnancy.
• Characterization of a conjugative transposon integrase, IntDOT.
• Comparison of morphological effects of PGE2 and TGFbeta on osteoclastogenesis induced by RANKL in mouse bone marrow cell cultures.
• Cooperation peaks at intermediate disturbance.
• Differences in vascular reactivity between men and women.
• Discrimination of prostate cancer cells by reflection mode FTIR photoacoustic spectroscopy.
• Dynamic programming algorithms for comparing multineuronal spike trains via cost-based metrics and alignments.
• Embryo culture medium: which is the best?
• Fatal pneumococcal meningitis: failure of acute care focus of medical practice.
• Hypoxic regulation of Id-1 and activation of the unfolded protein response are aberrant in neuroblastoma.
• In vitro combination characterization of the new anticancer plant drug beta-elemene with taxanes against human lung carcinoma.
• Incurring greater health care costs: risk stratification of employees with bipolar disorder.
• IntDOT interactions with core- and arm-type sites of the conjugative transposon CTnDOT.
• Interaction of the Gifsy-1 Xis protein with the Gifsy-1 attP sequence.
• Large-scale mapping and validation of Escherichia coli transcriptional regulation from a compendium of expression profiles.
• Levo-tetrahydropalmatine inhibits cocaine's rewarding effects: experiments with self-administration and brain-stimulation reward in rats.
• Optical artefacts in transflection mode FTIR microspectroscopic images of single cells on a biological support: the effect of back-scattering into collection optics.
• Previous traumatic birth: an impetus for requested cesarean birth.
• Selective leukemic-cell killing by a novel functional class of thalidomide analogs.
• Surviving change: Can the ESRD provisions in the SCHIP create a level playing field?
• The bacteroides NBU1 integrase performs a homology-independent strand exchange to form a holliday junction intermediate.
• The core role of the nurse practitioner: practice, professionalism and clinical leadership.
• The effect of current cigarette smoking on calf muscle hemoglobin oxygen saturation in patients with intermittent claudication.
• The influence of obesity on arterial compliance in adult men and women.
• The relationship between arterial elasticity and metabolic syndrome features.
• Transcriptional repression by a conserved intronic sequence in the nicotinic receptor alpha3 subunit gene.
• Understanding systemic sclerosis through gene expression profiling.
• Vitamin D activates type A natriuretic peptide receptor gene transcription in inner medullary collecting duct cells.