Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells.

Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells. Research Abstract Details 

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Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells. Abstract Text:

Masanori Imamura,Kyoko Miura,Kumiko Iwabuchi,Tomoko Ichisaka,Masato Nakagawa,Jiyoung Lee,Mito Kanatsu-Shinohara,Takashi Shinohara,Shinya Yamanaka,

BACKGROUND: We previously identified a set of genes called ECATs (ES cell-associated transcripts) that are expressed at high levels in mouse ES cells. Here, we examine the expression and DNA methylation of ECATs in somatic cells and germ cells. RESULTS: In all ECATs examined, the promoter region had low methylation levels in ES cells, but higher levels in somatic cells. In contrast, in spite of their lack of pluripotency, male germline stem (GS) cells expressed most ECATs and exhibited hypomethylation of ECAT promoter regions. We observed a similar hypomethylation of ECAT loci in adult testis and isolated sperm. Some ECATs were even less methylated in male germ cells than in ES cells. However, a few ECATs were not expressed in GS cells, and most of them targets of Oct3/4 and Sox2. The Octamer/Sox regulatory elements were hypermethylated in these genes. In addition, we found that GS cells express little Sox2 protein and low Oct3/4 protein despite abundant expression of their transcripts. CONCLUSION: Our results suggest that DNA hypermethylation and transcriptional repression of a small set of ECATs, together with post-transcriptional repression of Oct3/4 and Sox2, contribute to the loss of pluripotency in male germ cells.

Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells. Publishing Authors By Initials

M Imamura,K Miura,K Iwabuchi,T Ichisaka,M Nakagawa,J Lee,M Kanatsu-Shinohara,T Shinohara,S Yamanaka,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC developmental biology

VOLUME: 6

Page Numbers: 34

Journal Abbreviation: BMC Dev. Biol.

ISSN: 1471-213X

DAY: 21

MONTH: 07

YEAR: 2006

Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells. Information

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LANGUAGE: eng

NlmUniqueID: 100966973

Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells.

AFFILIATION: Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan. imamura@frontier.kyoto-u.ac.jp

Country: England

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MEDLINETA: BMC Dev Biol

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