Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts.

Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts. Research Abstract Details 

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Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts. Abstract Text:

R C Pereira,D Durant,E Canalis,

Glucocorticoids have important effects on osteoblastic function. Connective tissue growth factor (CTGF)/insulin-like growth factor binding protein-related protein 2 (IGFBP-rP2) plays a role in cell adhesion and function. We examined the regulation of CTGF/IGFBP-rP2 synthesis in cultures of osteoblast-enriched cells from 22-day fetal rat calvariae (Ob cells). Cortisol caused a time- and dose-dependent increase in CTGF/IGFBP-rP2 mRNA levels in Ob cells. Cycloheximide did not preclude the effect, indicating that it was not protein synthesis dependent. Cortisol increased the rate of CTGF/IGFBP-rP2 transcription and, in transcriptionally arrested Ob cells, did not modify the decay of the transcript. Parathyroid hormone decreased, whereas transforming growth factor-beta and, to a lesser extent, bone morphogenetic protein 2 increased CTGF/IGFBP-rP2 mRNA levels, but other hormones and growth factors had no effect. In conclusion, cortisol stimulates CTGF/IGFBP-rP2 transcription in Ob cells. Because CTGF/IGFBP-rP2 binds IGFs, its increased expression could be relevant to the actions of cortisol in bone.

Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts. Publishing Authors By Initials

RC Pereira,D Durant,E Canalis,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: American journal of physiology. Endocrinology and

VOLUME: 279

Page Numbers: E570-6

Journal Abbreviation: Am. J. Physiol. Endocrinol. Me

ISSN: 0193-1849

DAY: 19

MONTH: Sep

YEAR: 2000

Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901226

Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts. Information

Substance Name: Cycloheximide

Registry Number: 66-81-9

Grant and Affiliation Information for Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts.

AFFILIATION: Department of Research, Saint Francis Hospital and Medical Center, Hartford, CT 06105, USA.

Country: UNITED STATES

AGENCY: United States NIDDK

GRANT: DK-45227

ACRONYM: DK

MEDLINETA: Am J Physiol Endocrinol Metab

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