Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers.

Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers. Abstract Text:

Scott A Ochsner, Strick-Marchand,Qiong Qiu,Susan Venable,Adam Dean,Margaret Wilde,Mary C Weiss,Gretchen J Darlington,Scott A Ochsner, Strick-Marchand,Qiong Qiu,Susan Venable,Adam Dean,Margaret Wilde,Mary C Weiss,Gretchen J Darlington,Scott A Ochsner, Strick-Marchand,Qiong Qiu,Susan Venable,Adam Dean,Margaret Wilde,Mary C Weiss,Gretchen J Darlington,

The ability to purify to homogeneity a population of hepatic progenitor cells from adult liver is critical for their characterization prior to any therapeutic application. As a step in this direction, we have used a bipotential liver cell line from 14 days postcoitum mouse embryonic liver to compile a list of cell surface markers expressed specifically by liver progenitor cells. These cells, known as bipotential mouse embryonic liver (BMEL) cells, proliferate in an undifferentiated state and are capable of differentiating into hepatocyte-like and cholangiocyte-like cells in vitro. Upon transplantation, BMEL cells are capable of differentiating into hepatocytes and cholangiocytes in vivo. Microarray and Gene Ontology (GO) analysis of gene expression in the 9A1 and 14B3 BMEL cell lines grown under proliferating and differentiating conditions was used to identify cell surface markers preferentially expressed in the bipotential undifferentiated state. This analysis revealed that proliferating BMEL cells express many genes involved in cell cycle regulation, whereas differentiation of BMEL cells by cell aggregation causes a switch in gene expression to functions characteristic of mature hepatocytes. In addition, microarray data and protein analysis indicated that the Notch signaling pathway could be involved in maintaining BMEL cells in an undifferentiated stem cell state. Using GO annotation, a list of cell surface markers preferentially expressed on undifferentiated BMEL cells was generated. One marker, Cd24a, is specifically expressed on progenitor oval cells in livers of diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate-treated animals. We therefore consider Cd24a expression a candidate molecule for purification of hepatic progenitor cells. Disclosure of potential conflicts of interest is found at the end of this article.

Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers. Publishing Authors By Initials

SA Ochsner,H Strick-Marchand,Q Qiu,S Venable,A Dean,M Wilde,MC Weiss,GJ Darlington,SA Ochsner,H Strick-Marchand,Q Qiu,S Venable,A Dean,M Wilde,MC Weiss,GJ Darlington,SA Ochsner,H Strick-Marchand,Q Qiu,S Venable,A Dean,M Wilde,MC Weiss,GJ Darlington,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Stem cells (Dayton, Ohio)

VOLUME: 25

Page Numbers: 2476-87

Journal Abbreviation: Stem Cells

ISSN: 1549-4918

DAY: 19

MONTH: 07

YEAR: 2007

Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9304532

Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers.

AFFILIATION: Huffington Center on Aging, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: U01 DK63588

ACRONYM: DK

MEDLINETA: Stem Cells

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers Related Publications

• Adenosine and cytokine levels following treatment of rheumatoid arthritis with dipyridamole.
• Alterations in xenobiotic metabolism in the long-lived Little mice.
• Blood and speech.
• Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes.
• Cytosolic glucocorticoid receptor expression in the rat vestibular nucleus and hippocampus following unilateral vestibular deafferentation.
• Effects of intra-vestibular nucleus injection of the group I metabotropic glutamate receptor antagonist AIDA on vestibular compensation in guinea pigs.
• Genetic particles.
• Glutamate receptor subunits expression in memory-associated brain structures: Regional variations and effects of aging.
• Inflammatory status and kynurenine metabolism in rheumatoid arthritis treated with melatonin.
• Lymphoid neogenesis and immune infiltration in aged liver.
• MBP-8298, a synthetic peptide analog of myelin basic protein for the treatment of multiple sclerosis.
• Medical history, sexual, and maturational factors and prostate cancer risk.
• Oral tumors.
• Osteonecrosis at multiple sites in a patient with systemic lupus erythematosus.
• Overview of research on health-related quality of life in patients with chronic liver disease.
• Quantitative changes in gene expression of glutamate receptor subunits/subtypes in the vestibular nucleus, inferior olive and flocculus before and following unilateral labyrinthectomy in the rat: real-time quantitative PCR method.
• Reproductive versatility in Rubus; raspberry-blackberry hybrids; on an integrated species difference.
• The changing face of insulin receptors.
• The contribution of nitric oxide to vestibular compensation: are there species differences?
• The effect of delta 9-tetrahydrocannabinol on the extinction of an adverse associative memory.
• The geographical distribution of cold-blooded vertebrates.
• The geographical distribution of cold-blooded vertebrates.
• The plasmagene theory of the origin of cancer.
• The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain.
• Threat of disease in tropical crops.
• Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers.
• Tryptophan, adenosine, neurodegeneration and neuroprotection.