Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis.

Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis. Abstract Text:

Surendranath Baliji,Janet Sunter,Garry Sunter,

Spinach curly top virus (SCTV), the fifth characterized Curtovirus species belonging to the family Geminiviridae, is an agriculturally significant plant pathogen representing an emerging disease threat in the southern United States. The SCTV genome comprises a single DNA chromosome of approximately 3.0 kb, with the potential to code for seven proteins larger than 10 kDa but which relies extensively on the host for replication and transcription of its genome. In this study, we have identified viral and complementary sense transcripts in SCTV-infected plants, confirming a bidirectional transcription strategy for SCTV. The most abundant RNA maps to the virion sense (1.1-kb transcript) and is comparable in size and location to that observed in Beet curly top virus (BCTV). Two complementary sense transcripts (1.7 and 0.7 kb) were identified in SCTV-infected plants. The large, 1.7-kb transcript is comparable in size and position to that identified in BCTV and several begomoviruses and most likely encodes the C1 protein. Both complementary sense RNAs could potentially direct expression of C2 and C3 from polycistronic mRNAs. A mutation in the C2 gene of SCTV results in expression of a truncated protein of 38 amino acids that is capable of interacting with two cellular kinases, AKIN11 and ADK2, and the resulting mutant virus remains highly infectious. A second mutant virus can only express the first three amino acids of the C2 protein and is unable to interact with the same kinases. However, this mutant virus still remains infectious, although a reduction in infectivity and symptom severity was seen in both Arabidopsis and spinach. A possible relationship between the interaction of C2 with AKIN11 and ADK2 and disease severity is presented.

Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis. Publishing Authors By Initials

S Baliji,J Sunter,G Sunter,

For similar viruses: virion research abstracts see: viruses: virion research

PUBMED ID PMID:

MEDLINE DATE:

Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular plant-microbe interactions : MPMI

VOLUME: 20

Page Numbers: 194-206

Journal Abbreviation: Mol. Plant Microbe Interact.

ISSN: 0894-0282

DAY: 3

MONTH: Feb

YEAR: 2007

Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9107902

Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: growth & development

Chemical & Substance for Abstract: Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis. Information

Substance Name: Viral Proteins

Registry Number: 0

Grant and Affiliation Information for Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis.

AFFILIATION: Department of Biology, The University of Texas at San Antonio, San Antonio, TX 78249, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM-08194

ACRONYM: GM

MEDLINETA: Mol Plant Microbe Interact

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis Related Publications

• AL1-dependent repression of transcription enhances expression of Tomato golden mosaic virus AL2 and AL3.
• Cell proliferation in gastrointestinal carcinogenesis.
• Comprehensive care.
• Distinct viral sequence elements are necessary for expression of Tomato golden mosaic virus complementary sense transcripts that direct AL2 and AL3 gene expression.
• Functional analysis of bipartite begomovirus coat protein promoter sequences.
• Transcriptional analysis of complementary sense genes in Spinach curly top virus and functional role of C2 in pathogenesis.