Transcriptional activation of the melanocyte-specific genes by the human homolog of the mouse Microphthalmia protein.

Transcriptional activation of the melanocyte-specific genes by the human homolog of the mouse Microphthalmia protein. Research Abstract Details 

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Transcriptional activation of the melanocyte-specific genes by the human homolog of the mouse Microphthalmia protein. Abstract Text:

K Yasumoto,H Mahalingam,H Suzuki,M Yoshizawa,K Yokoyama,

Mi protein encoded at the mouse microphthalmia (mi) locus is a transcription factor with a basic helix-loop-helix/leucine zipper structure. To assess the function of the human homolog of Mi protein, termed microphthalmia-associated transcription factor (MITF), we analyzed the effects of MITF on the promoter function of the mouse tyrosinase and tyrosinase-related protein 1 (TRP-1) genes. These two gene promoters are able to direct transcription preferentially in melanin-producing cells, and an enhancer element M box of 11 bp, containing a CATGTG motif, is conserved in both promoters. By transient expression assays, we have localized the cis-acting element of the tyrosinase gene responsible for pigment cell-specific expression to the proximal 82-bp region, which contains a CATGTG motif (positions -12 to -7) but lacks the M box (positions -107 to -97). We also provide evidence that the 82-bp region and the M box are involved in the transactivation of the tyrosinase promoter by MITF and that the M box is bound by MITF in vitro. Furthermore, MITF activated the TRP-1 gene promoter possibly through the M box (positions -44 to -34). These results suggest that MITF is a common factor regulating transcription of the pigment cell-specific genes.

Transcriptional activation of the melanocyte-specific genes by the human homolog of the mouse Microphthalmia protein. Publishing Authors By Initials

K Yasumoto,H Mahalingam,H Suzuki,M Yoshizawa,K Yokoyama,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Transcriptional activation of the melanocyte-specific genes by the human homolog of the mouse Microphthalmia protein. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 118

Page Numbers: 874-81

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1995

Transcriptional activation of the melanocyte-specific genes by the human homolog of the mouse Microphthalmia protein. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Transcriptional activation of the melanocyte-specific genes by the human homolog of the mouse Microphthalmia protein. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: genetics

Chemical & Substance for Abstract: Transcriptional activation of the melanocyte-specific genes by the human homolog of the mouse Microphthalmia protein. Information

Substance Name: Glutathione Transferase

Registry Number: EC 2.5.1.18

Grant and Affiliation Information for Transcriptional activation of the melanocyte-specific genes by the human homolog of the mouse Microphthalmia protein.

AFFILIATION: Department of Applied Physiology and Molecular Biology, Tohoku University School of Medicine.

Country: JAPAN

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MEDLINETA: J Biochem

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