Transcription of mouse ribosomal RNA gene with inactive extracts is activated by NAD+ in vitro.

Transcription of mouse ribosomal RNA gene with inactive extracts is activated by NAD+ in vitro. Research Abstract Details 

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Transcription of mouse ribosomal RNA gene with inactive extracts is activated by NAD+ in vitro. Abstract Text:

Y Mishima,T Nishimura,M Muramatsu,R Kominami,

S100 extract prepared from rapidly growing mouse FM3A cells (approx. 5 x 10(5) cells/ml) transcribed ribosomal RNA gene (rDNA) much more actively in vitro than that from stationary phase cells (1-2 x 10(6) cells/ml). When the inactive S100 extract was preincubated with NAD+, rDNA transcriptional activity was restored almost to the level of the active extract. The extract activated with NAD+ exhibited a gel-shift band in the gel mobility shift assay and enhancement of protection of the sequence between -44 and -8 nt from the initiation site from exonuclease III digestion. Such an extract labeled with [32P]NAD+ was analyzed by immunoprecipitation with anti-RNA polymerase I (pol I) antibody; a protein with M(r) 130 kDa was detected. In contrast, the polypeptide was hardly labeled in the active extract. 3-Aminobenzamide, a specific inhibitor of poly ADP-ribosylation, did not inhibit the activation by NAD+. These results suggest that the activation by NAD+ is due to enhancement of the formation of initiation complex by mono ADP-ribosylation of the second-largest subunit (130 kDa) of pol I.

Transcription of mouse ribosomal RNA gene with inactive extracts is activated by NAD+ in vitro. Publishing Authors By Initials

Y Mishima,T Nishimura,M Muramatsu,R Kominami,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Transcription of mouse ribosomal RNA gene with inactive extracts is activated by NAD+ in vitro. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 113

Page Numbers: 36-42

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1993

Transcription of mouse ribosomal RNA gene with inactive extracts is activated by NAD+ in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Transcription of mouse ribosomal RNA gene with inactive extracts is activated by NAD+ in vitro. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Transcription of mouse ribosomal RNA gene with inactive extracts is activated by NAD+ in vitro. Information

Substance Name: NAD

Registry Number: 53-84-9

Grant and Affiliation Information for Transcription of mouse ribosomal RNA gene with inactive extracts is activated by NAD+ in vitro.

AFFILIATION: Department of Biochemistry, Niigata University School of Medicine.

Country: JAPAN

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MEDLINETA: J Biochem

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