Tracking expression of virally mediated BMP-2 in gene therapy for bone repair.

Tracking expression of virally mediated BMP-2 in gene therapy for bone repair. Research Abstract Details 

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Tracking expression of virally mediated BMP-2 in gene therapy for bone repair. Abstract Text:

Seth C Gamradt,Nobuhiro Abe,Matthew E Bahamonde,Yu-Po Lee,Scott D Nelson,Karen M Lyons,Jay R Lieberman,

Ex vivo gene therapy using stem cells transduced with viral vectors is a useful method for delivering a therapeutic protein to augment bone repair in animal models. However, the duration of cell-mediated protein production and the fate of the transduced cells are unknown. We constructed an adenoviral vector encoding Myc epitope tagged bone morphogenetic protein (BMP)-2 gene (AdBMP-2). Rat bone marrow cells transduced with this vector produced biologically active BMP-2 protein, which was confirmed by Western blot analysis and alkaline phosphatase assay. Implantation of bone marrow cells infected ex vivo with AdBMP-2 caused orthotopic bone formation in mouse hindlimbs and bony union of critical-sized mouse radial defects. Immunohistochemical analysis revealed that rBMCs expressed Myc epitope-tagged BMP-2 protein for 14 days in vivo and became incorporated in the endochondral fracture callus. This novel adenovirus encoding for epitope-tagged BMP-2 can be used for immunohistochemical tracking of transduced cells in ex vivo gene therapy for bone repair.

Tracking expression of virally mediated BMP-2 in gene therapy for bone repair. Publishing Authors By Initials

SC Gamradt,N Abe,ME Bahamonde,YP Lee,SD Nelson,KM Lyons,JR Lieberman,

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Tracking expression of virally mediated BMP-2 in gene therapy for bone repair. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Clinical orthopaedics and related research

VOLUME: 450

Page Numbers: 238-45

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ISSN: 0009-921X

DAY: 19

MONTH: Sep

YEAR: 2006

Tracking expression of virally mediated BMP-2 in gene therapy for bone repair. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Tracking expression of virally mediated BMP-2 in gene therapy for bone repair.

AFFILIATION: Department of Orthopaedic Surgery, School of Medicine, University of California-Los Angeles Medical Center, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA. sgamradt@mednet.ucla.edu

Country: United States

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MEDLINETA: Clin Orthop Relat Res

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