Total modification of the bacteriophage lambda tail tube major subunit protein with foreign peptides.

Total modification of the bacteriophage lambda tail tube major subunit protein with foreign peptides. Research Abstract Details 

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Total modification of the bacteriophage lambda tail tube major subunit protein with foreign peptides. Abstract Text:

I S Dunn,

The bacteriophage lambda has been shown previously to tolerate a high multiplicity of peptide additions to the C-terminus of the major tail tube subunit protein (gpV, the product of the V gene). However, it was not clear whether all gpV copies within a functional virion could tolerate such modification. Complementation tests with phage bearing either V gene amber mutations or a precisely deleted V gene were used to test the extent of possible tail tube peptide display. Expression of plasmid-encoded gpV fused C-terminally with certain foreign peptides allowed rescue of such V gene-defective phage to essentially wild-type levels. After extensive purification such phage were shown by sensitive Western blotting to contain only the modified form of gpV. Peptide-modified gpV could also form indefinite tail tube polymeric structures (polytubes).

Total modification of the bacteriophage lambda tail tube major subunit protein with foreign peptides. Publishing Authors By Initials

IS Dunn,

For similar proteins: viral proteins: viral structural proteins: viral tail proteins research abstracts see: proteins: viral proteins: viral structural proteins: viral tail proteins research

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Total modification of the bacteriophage lambda tail tube major subunit protein with foreign peptides. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Gene

VOLUME: 183

Page Numbers: 15-21

Journal Abbreviation: Gene

ISSN: 0378-1119

DAY: 12

MONTH: Dec

YEAR: 1996

Total modification of the bacteriophage lambda tail tube major subunit protein with foreign peptides. Information

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LANGUAGE: eng

NlmUniqueID: 7706761

Total modification of the bacteriophage lambda tail tube major subunit protein with foreign peptides. Keywords Mesh Terms:

KEYWORDS: Viral Tail Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Total modification of the bacteriophage lambda tail tube major subunit protein with foreign peptides. Information

Substance Name: maltoporins

Registry Number: 0

Grant and Affiliation Information for Total modification of the bacteriophage lambda tail tube major subunit protein with foreign peptides.

AFFILIATION: Queensland Cancer Fund Research Unit, Department of Pathology, Medical School, University of Queensland, Herston, Brisbane, Australia. iand@mail.kids.usyd.edu.au

Country: NETHERLANDS

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MEDLINETA: Gene

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