Total internal reflection with fluorescence correlation spectroscopy.

Total internal reflection with fluorescence correlation spectroscopy. Research Abstract Details 

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Total internal reflection with fluorescence correlation spectroscopy. Abstract Text:

Nancy L Thompson,Bridgett L Steele,Nancy L Thompson,Bridgett L Steele,

Total internal reflection-fluorescence correlation spectroscopy (TIR-FCS) is an emerging technique that is used to measure events at or near an interface, including local fluorophore concentrations, local translational mobilities and the kinetic rate constants that describe the association and dissociation of fluorophores at the interface. TIR-FCS is also an extremely promising method for studying dynamics at or near the basal membranes of living cells. This protocol gives a general overview of the steps necessary to construct and test a TIR-FCS system using either through-prism or through-objective internal reflection geometry adapted for FCS. The expected forms of the autocorrelation function are discussed for the cases in which fluorescent molecules in solution diffuse through the depth of the evanescent field, but do not bind to the surface of interest, and in which reversible binding to the surface also occurs.

Total internal reflection with fluorescence correlation spectroscopy. Publishing Authors By Initials

NL Thompson,BL Steele,NL Thompson,BL Steele,

For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

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Total internal reflection with fluorescence correlation spectroscopy. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nature protocols

VOLUME: 2

Page Numbers: 878-90

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2007

Total internal reflection with fluorescence correlation spectroscopy. Information

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LANGUAGE: eng

NlmUniqueID: 101284307

Total internal reflection with fluorescence correlation spectroscopy. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Fluorescence

MESH TERMS: methods

Chemical & Substance for Abstract: Total internal reflection with fluorescence correlation spectroscopy. Information

Substance Name: Ligands

Registry Number: 0

Grant and Affiliation Information for Total internal reflection with fluorescence correlation spectroscopy.

AFFILIATION: Department of Chemistry, University of North Carolina at Chapel Hill, North Carolina 27599, USA. nlt@unc.edu

Country: England

AGENCY: United States NHLBI

GRANT: HL073937

ACRONYM: HL

MEDLINETA: Nat Protoc

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