Topology of the mammalian cell via cryo-FIB etching.

Topology of the mammalian cell via cryo-FIB etching. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Topology of the mammalian cell via cryo-FIB etching. Abstract Text:

J E M McGeoch,

Mapping of a mammalian cell down to a feature size of 20-30 nm in 3D is a goal that will answer many questions concerning the connectivity (topology) of a Eukaryotic cell's traffic routes. These routes are defined and separated from one another by the protein-impregnated lipid membrane barrier of the endoplasmic reticulum (ER). We trace the routes from outside a live flash frozen buccal epithelial cell via gold (Au) labelled pores in the plasma membrane to the ER below and then through the cell as isosurfaces in 3D maps. The outer tubular ER with three-way branching changes to a sheet-like ER nearer the nucleus, and the cytoplasmic space between the ER membranes continues as a volume into the nuclear interior via the nuclear pores. We find some evidence that the last layer of the cytoplasmic ER membrane, also termed the outer nuclear membrane, has discrete gaps, so the ER lumen in these areas is continuous with the nuclear luminal domain and further, the inner nuclear membrane has small protrusions into the nucleus. The routes were established in live, unstained, unfixed, cells etched with a pAmp current of a focused ion beam (cryo-FIB) dual beam electron microscope, at -150 degrees C, 1e-4Pa, and confirmed at 37 degrees C in lipid-dye stained cells. The cryo-FIB etch of a cuboid of 2D planes, and its reconstruction into many 3D maps, takes only hours, facilitating the execution of experiments with comparative conditions in a few days.

Topology of the mammalian cell via cryo-FIB etching. Publishing Authors By Initials

JE McGeoch,

For similar diagnosis: diagnostic techniques and procedures: diagnostic imaging: microscopy: microscopy, fluorescence research abstracts see: diagnosis: diagnostic techniques and procedures: diagnostic imaging: microscopy: microscopy, fluorescence research

PUBMED ID PMID:

MEDLINE DATE:

Topology of the mammalian cell via cryo-FIB etching. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of microscopy

VOLUME: 227

Page Numbers: 172-84

Journal Abbreviation:

ISSN: 0022-2720

DAY: 30

MONTH: Aug

YEAR: 2007

Topology of the mammalian cell via cryo-FIB etching. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 204522

Topology of the mammalian cell via cryo-FIB etching. Keywords Mesh Terms:

KEYWORDS: Microscopy, Fluorescence

MESH TERMS: anatomy & histology

Chemical & Substance for Abstract: Topology of the mammalian cell via cryo-FIB etching. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Topology of the mammalian cell via cryo-FIB etching.

AFFILIATION: Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA. mcgeoch@fas.harvard.edu

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Microsc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Topology of the mammalian cell via cryo-FIB etching Related Publications

• A new treatment for chronic itch?
• Block to influenza virus replication in cells preirradiated with ultraviolet light.
• Entrapment of water by subunit c of ATP synthase.
• Novel Mammalian Herpesviruses and Lineages within the Gammaherpesvirinae: Cospeciation and Interspecies Transfer.
• Occurrence of phantom genitalia after gender reassignment surgery.
• Radio-frequency-preionized xenon z-pinch source for extreme ultraviolet lithography.
• Shakespeare the dermatologist.
• Spatial variation in plant interactions across a severity gradient in the sub-Antarctic.
• Topology of the mammalian cell via cryo-FIB etching.