Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I).

Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I). Research Abstract Details 

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Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I). Abstract Text:

Irina S Gostimskaya,Gary Cecchini,Andrei D Vinogradov,Irina S Gostimskaya,Gary Cecchini,Andrei D Vinogradov,

The spatial arrangement and chemical reactivity of the activation-dependent thiol in the mitochondrial Complex I was studied using the membrane penetrating N-ethylmaleimide (NEM) and non-penetrating anionic 5,5'-dithiobis-(2-nitrobenzoate) (DTNB) as the specific inhibitors of the enzyme in mitochondria and inside-out submitochondrial particles (SMP). Both NEM and DTNB rapidly inhibited the de-activated Complex I in SMP. In mitochondria NEM caused rapid inhibition of Complex I, whereas the enzyme activity was insensitive to DTNB. In the presence of the channel-forming antibiotic alamethicin, mitochondrial Complex I became sensitive to DTNB. Neither active nor de-activated Complex I in SMP was inhibited by oxidized glutathione (10 mM, pH 8.0, 75 min). The data suggest that the active/de-active transition sulfhydryl group of Complex I which is sensitive to inhibition by NEM is located at the inner membrane-matrix interface. These data include the sidedness dependency of inhibition, effect of pH, ionic strength, and membrane bilayer modification on enzyme reactivity towards DTNB and its neutral analogue.

Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I). Publishing Authors By Initials

IS Gostimskaya,G Cecchini,AD Vinogradov,IS Gostimskaya,G Cecchini,AD Vinogradov,

For similar sulfhydryl compounds research abstracts see: sulfhydryl compounds research

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Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I). Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biochimica et biophysica acta

VOLUME: 1757

Page Numbers: 1155-61

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 4

MONTH: 05

YEAR: 2006

Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 217513

Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I). Keywords Mesh Terms:

KEYWORDS: Sulfhydryl Compounds

MESH TERMS: metabolism

Chemical & Substance for Abstract: Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I). Information

Substance Name: Electron Transport Complex I

Registry Number: EC 1.6.5.3

Grant and Affiliation Information for Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I).

AFFILIATION: Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russian Federation.

Country: Netherlands

AGENCY: United States FIC

GRANT: 1R03TW006041

ACRONYM: TW

MEDLINETA: Biochim Biophys Acta

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