Tooth slice-based models for the study of human dental pulp angiogenesis.

Tooth slice-based models for the study of human dental pulp angiogenesis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Tooth slice-based models for the study of human dental pulp angiogenesis. Abstract Text:

Silvana B ,Zhihong Dong,Clovis M Bramante,Graham R Holland,Anthony J Smith,Jacques E ,

Treatment of avulsed young permanent teeth aims to revascularize the dental pulp. The study of therapeutic strategies for avulsed teeth has been hindered by the scarcity of experimental models. The purpose of this work is to characterize two model systems to study dental pulp revascularization. Tooth slices from human third molars were prepared with a sterile diamond saw. The tooth slices were cultured in vitro for up to 7 days. Immunohistochemical staining with Factor VIII showed an increase in microvascular density in pulps treated with 50 ng/mL rhVEGF(165) as compared with untreated controls (p < 0.05). Alternatively, tooth slices were prepared and immediately implanted subcutaneously in immunodeficient mice. Pulp vitality and vascularization were confirmed by histological analysis and terminal deoxynucleotide transferase dUTP nick end labeling assays 7 days after implantation. The models presented here may be valuable in the assessment of angiogenesis-based therapeutic strategies for the dental pulp.

Tooth slice-based models for the study of human dental pulp angiogenesis. Publishing Authors By Initials

SB ,Z Dong,CM Bramante,GR Holland,AJ Smith,JE ,

For similar peptides: intercellular signaling peptides and proteins: angiogenic proteins: vascular endothelial growth factors: vascular endothelial growth factor a research abstracts see: peptides: intercellular signaling peptides and proteins: angiogenic proteins: vascular endothelial growth factors: vascular endothelial growth factor a research

PUBMED ID PMID:

MEDLINE DATE:

Tooth slice-based models for the study of human dental pulp angiogenesis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of endodontics

VOLUME: 33

Page Numbers: 811-4

Journal Abbreviation:

ISSN: 0099-2399

DAY: 7

MONTH: 05

YEAR: 2007

Tooth slice-based models for the study of human dental pulp angiogenesis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7511484

Tooth slice-based models for the study of human dental pulp angiogenesis. Keywords Mesh Terms:

KEYWORDS: Vascular Endothelial Growth Factor A

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Tooth slice-based models for the study of human dental pulp angiogenesis. Information

Substance Name: Factor VIII

Registry Number: 9001-27-8

Grant and Affiliation Information for Tooth slice-based models for the study of human dental pulp angiogenesis.

AFFILIATION: Department of Cariology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Endod

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Tooth slice-based models for the study of human dental pulp angiogenesis Related Publications

• Antiangiogenic effect of TW37, a small-molecule inhibitor of Bcl-2.
• Bcl-2 orchestrates a cross-talk between endothelial and tumor cells that promotes tumor growth.
• Cancer gene therapy with iCaspase-9 transcriptionally targeted to tumor endothelial cells.
• Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth.
• Effect of ProRoot MTA mixed with chlorhexidine on apoptosis and cell cycle of fibroblasts and macrophages in vitro.
• Endothelial Cells Enhance Tumor Cell Invasion through a Crosstalk Mediated by CXC Chemokine Signaling.
• Expanding circle of inhibition: small-molecule inhibitors of bcl-2 as anticancer cell and antiangiogenic agents.
• Level of endothelial cell apoptosis required for a significant decrease in microvessel density.
• Quantification of human angiogenesis in immunodeficient mice using a photon counting-based method.
• Tooth slice-based models for the study of human dental pulp angiogenesis.
• Unidirectional crosstalk between Bcl-xL and Bcl-2 enhances the angiogenic phenotype of endothelial cells.