Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney.

Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney. Research Abstract Details 

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Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney. Abstract Text:

S Yoshimura,K Watanabe,H Suemizu,T Onozawa,J Mizoguchi,K Tsuda,H Hatta,T Moriuchi,

Rat plasma glutathione peroxidase (GSH-Px) was purified 1,400-fold from rat serum by a combination of phenyl Sepharose, DEAE Sephacel, blue Sepharose and Sephacryl S-200 column chromatographies. The purified GSH-Px migrated as a single band corresponding to a molecular weight of 22,500 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme was used for the immunization of chickens to obtain a specific antibody and for determination of its amino acid sequence. Two overlapping cDNA clones for rat plasma GSH-Px were isolated from a placental cDNA library. The composite nucleotide sequence is 1,529 base-pairs long and encodes 226 amino acids. The deduced amino acid sequence completely coincided with the sequences of five individual peptide fragments derived from the purified plasma GSH-Px on digestion with lysyl endopeptidase. In order to identify the tissue(s) generating this plasma GSH-Px, immunoblot analysis was performed on homogenates prepared from 13 tissues. A single immunoreactive band of 22.5 kDa, corresponding to plasma GSH-Px, was detected for the kidney homogenate. A much fainter band was observed for the lung preparation, but liver, spleen, bone marrow, and other tissues examined were negative. Northern blot analysis further revealed that the expression level of the plasma GSH-Px gene was high in kidney and low in lung. No transcript was detected in liver or spleen. These results indicate that plasma GSH-Px is predominantly synthesized and secreted by renal cells.

Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney. Publishing Authors By Initials

S Yoshimura,K Watanabe,H Suemizu,T Onozawa,J Mizoguchi,K Tsuda,H Hatta,T Moriuchi,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains research

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Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 109

Page Numbers: 918-23

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1991

Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred Strains

MESH TERMS: enzymology

Chemical & Substance for Abstract: Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney. Information

Substance Name: Glutathione Peroxidase

Registry Number: EC 1.11.1.9

Grant and Affiliation Information for Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney.

AFFILIATION: Department of Cell Biology, Tokai University School of Medicine, Kanagawa.

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: S69830

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