Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue.

Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue. Research Abstract Details 

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Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue. Abstract Text:

Theodore P Ciaraldi,Deborah K Oh,Louis Christiansen,Svetlana E Nikoulina,Alice P S Kong,Sunita Baxi,Sunder Mudaliar,Robert R Henry,

Glycogen synthase kinase-3 (GSK-3) is a ubiquitous kinase implicated in both insulin action and adipogenesis. To determine how these multiple roles may relate to insulin resistance, we studied the regulation of GSK-3 protein expression and phosphorylation in skeletal muscle and isolated adipocytes from nonobese healthy control (HC), obese control (OC), and obese type 2 diabetic (OT2D) subjects. At baseline there were no differences in the GSK-3 protein expression in adipocytes. OC subjects underwent a 6-mo caloric restriction resulting in a 7% decrease in body mass index (BMI) and a 21% improvement in insulin-stimulated whole body glucose disposal rate (GDR). GSK-3alpha and GSK-3beta expression decreased in adipocytes (P < 0.05), whereas GSK-3alpha protein expression increased in skeletal muscle (P < 0.05). OT2D subjects were treated with troglitazone or metformin for 3-4 mo. After troglitazone treatment GDR improved (P < 0.05) despite an increase in BMI (P < 0.05), whereas metformin had no significant effect on GDR. There was no significant change in GSK-3 expression in adipocytes following troglitazone, whereas both GSK-3alpha and -beta were decreased in skeletal muscle (P < 0.05). Metformin treatment had no significant impact on GSK-3 protein expression in either adipocytes or skeletal muscle. Neither treatment influenced GSK-3 serine phosphorylation in skeletal muscle or adipocytes. These results suggest that there is tissue specificity for the regulation of GSK-3 in humans. In skeletal muscle GSK-3 plays a role in control of metabolism and insulin action, whereas the function in adipose tissue is less clear.

Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue. Publishing Authors By Initials

TP Ciaraldi,DK Oh,L Christiansen,SE Nikoulina,AP Kong,S Baxi,S Mudaliar,RR Henry,

For similar pathological conditions, signs and symptoms: signs and symptoms: body weight: body weight changes: weight loss research abstracts see: pathological conditions, signs and symptoms: signs and symptoms: body weight: body weight changes: weight loss research

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Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: American journal of physiology. Endocrinology and

VOLUME: 291

Page Numbers: E891-8

Journal Abbreviation: Am. J. Physiol. Endocrinol. Me

ISSN: 0193-1849

DAY: 6

MONTH: 06

YEAR: 2006

Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901226

Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue. Keywords Mesh Terms:

KEYWORDS: Weight Loss

MESH TERMS: administration & dosage

Chemical & Substance for Abstract: Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue. Information

Substance Name: glycogen synthase kinase 3 beta

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue.

AFFILIATION: Veterans Affairs San Diego Healthcare System, San Diego, CA 92161, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01-DK-258291

ACRONYM: DK

MEDLINETA: Am J Physiol Endocrinol Metab

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