Tissue-preserving approach to extracting DNA from paraffin-embedded specimens using tissue microarray technology.

Tissue-preserving approach to extracting DNA from paraffin-embedded specimens using tissue microarray technology. Research Abstract Details 

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Tissue-preserving approach to extracting DNA from paraffin-embedded specimens using tissue microarray technology. Abstract Text:

Mark E Prince,Mathew L Ubell,Jason Castro,Haruko Ogawa,Tetsuya Ogawa,Ajita Narayan,Augusto Paulino,Andrew Cole,Gregory T Wolf,Mark A Rubin,Robert H Lyons,Carol R Bradford,Thomas E Carey,

BACKGROUND: DNA extracted from tumor cells or normal cells contained in formalin-fixed, paraffin-embedded tissues is widely used in many laboratories. The 2 most common procedures to isolate cells for DNA extraction from paraffin-embedded tissues are scalpel microdissection and laser capture microdissection. A new tissue- and time-conserving method for rapid DNA isolation from small cores taken from paraffin-embedded tissue blocks is described in this report. METHODS: DNA was extracted from small tissue cores collected from paraffin-embedded tissue blocks at the time of tissue microarray construction. The quality and quantity of the DNA extracted was compared to DNA collected by scalpel microdissection. DNA collected from tissue cores was used in polymerase chain reaction (PCR) and loss of heterozygosity (LOH) analysis. RESULTS: The quality and quantity of DNA obtained using tissue cores was comparable to DNA obtained by traditional methods. The tissue core method of DNA extraction preserves the tissue blocks from which the cores are extracted for future use. Adequate quantities of DNA can be successfully extracted from small segments of tissue cores and used for PCR. DNA isolated by tissue microdissection and the tissue core method were comparable when used to assess allelic heterozygosity on chromosome arm 18q. CONCLUSION: The tissue core method of DNA isolation is reliable, tissue conserving, and time effective. Tissue cores for DNA extraction can be harvested at the same time as tissue microarray construction. The technique has the advantage of preserving the original tissue blocks for additional study as only tiny cores are removed.

Tissue-preserving approach to extracting DNA from paraffin-embedded specimens using tissue microarray technology. Publishing Authors By Initials

ME Prince,ML Ubell,J Castro,H Ogawa,T Ogawa,A Narayan,A Paulino,A Cole,GT Wolf,MA Rubin,RH Lyons,CR Bradford,TE Carey,

For similar investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: tissue array analysis research abstracts see: investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: tissue array analysis research

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Tissue-preserving approach to extracting DNA from paraffin-embedded specimens using tissue microarray technology. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Head & neck

VOLUME: 29

Page Numbers: 465-71

Journal Abbreviation:

ISSN: 1043-3074

DAY: 3

MONTH: May

YEAR: 2007

Tissue-preserving approach to extracting DNA from paraffin-embedded specimens using tissue microarray technology. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8902541

Tissue-preserving approach to extracting DNA from paraffin-embedded specimens using tissue microarray technology. Keywords Mesh Terms:

KEYWORDS: Tissue Array Analysis

MESH TERMS: pathology

Chemical & Substance for Abstract: Tissue-preserving approach to extracting DNA from paraffin-embedded specimens using tissue microarray technology. Information

Substance Name: DNA, Neoplasm

Registry Number: 0

Grant and Affiliation Information for Tissue-preserving approach to extracting DNA from paraffin-embedded specimens using tissue microarray technology.

AFFILIATION: Department of Otolaryngology-HNS, University of Michigan, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA.

Country: United States

AGENCY: United States NIDCD

GRANT: T32 DC 05356

ACRONYM: DC

MEDLINETA: Head Neck

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