Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model.

Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model. Abstract Text:

Jodie E Moreau,Kristen Anderson,Joshua R Mauney,Trang Nguyen,David L Kaplan,Michael Rosenblatt,

The high frequency and mortality associated with breast cancer metastasis to bone has motivated efforts to elucidate tumor-stroma interactions in the bone microenvironment contributing to invasion and proliferation of metastatic cells. The development of engineered tissues has prompted the integration of engineered bone scaffolds into animal models as potential targets for metastatic spread. Silk scaffolds were coupled with bone morphogenetic protein-2 (BMP-2), seeded with bone marrow stromal cells (BMSC), and maintained in culture for 7 weeks, 4 weeks, and 1 day before s.c. implant in a mouse model of human breast cancer metastasis from the orthotopic site. Following injection of SUM1315 cells into mouse mammary fat pads, tumor burden of implanted tissues was observed only in 1-day scaffolds. Scaffold development and implantation was then reinitiated to identify the elements of the engineered bone that contribute to metastatic spread. Untreated scaffolds were compared with BMP-2-coupled, BMSC-seeded, or BMP-2/BMSC-combined treatment. Migration of SUM1315 cells was detected in four of four mice bearing scaffolds with BMP-2 treatment and with BMSC treatment, respectively, whereas only one of six mice of the BMP-2/BMSC combination showed evidence of metastatic spread. Histology confirmed active matrix modeling and stromal cell/fibroblast infiltration in scaffolds positive for the presence of metastasis. These results show the first successful integration of engineered tissues in a model system of human breast cancer metastasis. This novel platform now can be used in continued investigation of the bone environment and stem cell contributions to the process of breast cancer metastasis.

Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model. Publishing Authors By Initials

JE Moreau,K Anderson,JR Mauney,T Nguyen,DL Kaplan,M Rosenblatt,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cancer research

VOLUME: 67

Page Numbers: 10304-8

Journal Abbreviation: Cancer Res.

ISSN: 1538-7445

DAY: 1

MONTH: Nov

YEAR: 2007

Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2984705

Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model.

AFFILIATION: Department of Physiology, Tufts University School of Medicine, Boston, MA 02111, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Cancer Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model Related Publications

• Action of methyl fluoroacetate on respiration and potential of nerve.
• Adoptively transferred tumor-specific T cells stimulated ex vivo using herpes simplex virus amplicons encoding 4-1BBL persist in the host and show antitumor activity in vivo.
• Augmentation of anti-tumor responses of adoptively transferred CD8+T cells in the lymphopenic setting by HSV amplicon transduction.
• Comparison of rate of development and rate of change for benign and malignant breast calcifications at the lumpectomy bed.
• Cysteine at position 217 in the intracellular loop 1 plays a critical role in human PTH receptor type 1 membrane translocation and function.
• Effect of dementia and treatment of dementia on time to discharge from assisted living facilities: the Maryland assisted living study.
• Endovascular management of venous malformations.
• Fluorescence-based analysis of cellular protein lysate arrays using quantum dots.
• Gilbert Gottlieb: Intermediator between psychology and evolutionary biology.
• In situ and infiltrating ductal carcinoma arising in a breast hamartoma.
• Insomnia and daytime sleepiness in people with dementia residing in assisted living: findings from the Maryland Assisted Living Study.
• Interesting anecdotes cannot validate test results.
• Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells.
• Line spectral analysis for harmonizable processes.
• Management of the patient reporting an allergy to penicillin.
• Naturally occurring reverse tilt domains in a high-pretilt alignment nematic liquid crystal.
• Recombinant expression and purification of the N-terminal extracellular domain of the parathyroid hormone receptor.
• Serum biomarkers for Alzheimer's disease: proteomic discovery.
• Studies of osteotropism on both sides of the breast cancer-bone interaction.
• The incidence of hypertension in portal cirrhosis; a study of 80 necropsied cases of portal cirrhosis.
• The laboratory management of thromboembolic disease.
• The ultrasound-guided supraclavicular-interscalene (ugscis) block: a case report.
• Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model.
• Transgenic corneal neurofluorescence in mice: a new model for in vivo investigation of nerve structure and regeneration.
• Use of synthetic mesh in pelvic reconstructive surgery: a survey of attitudes and practice patterns of urogynecologists.
• Using mediation to manage conflict in care facilities.
• You Don't Have to be Famous: How to Write Your Life Story.
• [Not Available.]
• [Socioeconomic factors, nutritional risk, and enamel defects in children from João Pessoa, Paraíba State, Brazil.]
• [Vaginal cytology during the 2d and 3d trimester of pregnancy and its role in the early detection of fetal distress]