Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells.

Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. Research Abstract Details 

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Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. Abstract Text:

Giuliana Gobbi,Prisco Mirandola,Ivonne Sponzilli,Cristina Micheloni,Chiara Malinverno,Lucio Cocco,Marco Vitale,

Protein kinase C (PKC)-mediated intracellular signaling participates in several key steps of hematopoietic cell differentiation. The epsilon isoform of PKC has been associated with erythroid differentiation as well as with the early phases of megakaryocytic (MK) lineage commitment. Here, we worked on the hypothesis that PKCepsilon expression levels might be modulated during MK differentiation, with a specific role in the early as well as in the late phases of thrombopoiesis. We demonstrate that--at variance with the erythroid lineage development--PKCepsilon is completely downmodulated in TPO-induced CD34 cells from day 6 onward. The forced expression of PKCepsilon in the late phases of MK differentiation delays the phenotypic differentiation of progenitors likely via Bcl-xL upregulation. Moreover, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), known as a negative regulator of early erythroid expansion, is not apoptogenic for thrombopoietin-induced CD34 cells, but rather accelerates their maturation. However, PKCepsilon levels negatively interfere also with the effects of TRAIL in MK differentiation. PKCepsilon can therefore be considered a signaling intermediate whose expression levels are finely tuned, with a virtually opposite kinetic, in erythroid versus megakaryocytic lineages, to adequately respond to the signaling requirements of the specific hematopoietic lineage.

Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. Publishing Authors By Initials

G Gobbi,P Mirandola,I Sponzilli,C Micheloni,C Malinverno,L Cocco,M Vitale,

For similar peptides: intracellular signaling peptides and proteins: apoptosis regulatory proteins: proto-oncogene proteins c-bcl-2: bcl-x protein research abstracts see: peptides: intracellular signaling peptides and proteins: apoptosis regulatory proteins: proto-oncogene proteins c-bcl-2: bcl-x protein research

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Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Stem cells (Dayton, Ohio)

VOLUME: 25

Page Numbers: 2322-9

Journal Abbreviation: Stem Cells

ISSN: 1549-4918

DAY: 14

MONTH: 06

YEAR: 2007

Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. Information

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LANGUAGE: eng

NlmUniqueID: 9304532

Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. Keywords Mesh Terms:

KEYWORDS: bcl-X Protein

MESH TERMS: genetics

Chemical & Substance for Abstract: Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. Information

Substance Name: Protein Kinase C-epsilon

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells.

AFFILIATION: Department of Anatomy, Pharmacology & Forensic Medicine, Human Anatomy Section, University of Parma, Ospedale Maggiore, Via Gramsci, 14, I-43100 Parma, Italy.

Country: United States

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MEDLINETA: Stem Cells

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