Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes.

Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes. Research Abstract Details 

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Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes. Abstract Text:

Qizhi Yao,Linh X Doan,Rongxin Zhang,Uddalak Bharadwaj,Min Li,Changyi Chen,

Although thymosins have been demonstrated to have immunomodulatory effects, it is still not clear whether they could affect dendritic cells (DCs), the most professional antigen-presenting cells. The objective of this study was to determine the effect and potential mechanisms of thymosin-alpha1 (Talpha1) on DC differentiation and functional maturation. Human peripheral blood CD14(+) monocytes were purified by using a magnetic separation column and cultured with GM-CSF and IL-4 to differentiate into immature DCs (iDCs). In the presence of Talpha1, iDC surface markers CD40, CD80, MHC class I and class II molecules were significantly upregulated as measured by flow cytemotry analysis. However, Tbeta4 or Tbeta10 did not show these effects on iDCs. There was an approximately 30% reduction in antigen (FITC-conjugated dextran)-uptake by Talpha1-treated iDCs as compared with non-Talpha1-treated iDCs. In addition, Talpha1-treated matured DCs (mDCs) showed an increased stimulation of allogeneic CD3(+) T-cell proliferation as measured by a mixed-lymphocyte reaction assay. Talpha1-treated mDCs also increased the production of several Th1- and Th2-type cytokines as measured by a Bio-Plex cytokine assay. Furthermore, rapid activation of p38 MAPK and NFkappaB was seen in Talpha1-treated iDCs as measured by a Bio-Plex phosphoprotein assay. Thus, Talpha1 significantly enhances DC differentiation, activation, and functions from human peripheral blood CD14(+) monocytes possibly through a mechanism of the activation of p38 MAPK and NFkappaB pathways. This study provides a basis to further evaluate Talpha1 as a possible adjuvant for a DC-directed vaccine or therapy.

Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes. Publishing Authors By Initials

Q Yao,LX Doan,R Zhang,U Bharadwaj,M Li,C Chen,

For similar hormones, hormone substitutes, and hormone antagonists: hormones: thymus hormones: thymosin research abstracts see: hormones, hormone substitutes, and hormone antagonists: hormones: thymus hormones: thymosin research

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Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Immunology letters

VOLUME: 110

Page Numbers: 110-20

Journal Abbreviation: Immunol. Lett.

ISSN: 0165-2478

DAY: 15

MONTH: 05

YEAR: 2007

Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7910006

Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes. Keywords Mesh Terms:

KEYWORDS: Thymosin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes. Information

Substance Name: thymosin beta(10)

Registry Number: 87397-91-9

Grant and Affiliation Information for Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes.

AFFILIATION: Molecular Surgeon Research Center, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA. qizhiyao@bcm.edu <qizhiyao@bcm.edu>

Country: Netherlands

AGENCY: United States NHLBI

GRANT: HL72716

ACRONYM: HL

MEDLINETA: Immunol Lett

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