Three-dimensional particle tracking via bifocal imaging.

Three-dimensional particle tracking via bifocal imaging. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Three-dimensional particle tracking via bifocal imaging. Abstract Text:

Erdal Toprak,Hamza Balci,Benjamin H Blehm,Paul R Selvin,

We introduce a bifocal imaging method that enables three-dimensional (3D) tracking of both fluorescent and nonfluorescent particles. We accomplish this by simultaneously imaging a focused plane, for in-plane position (x,y), and a defocused plane, for out-of-plane position (z), of a molecule using a single CCD camera. We applied our method to several systems including in vivo melanosome tracking and phagocytosed fluorescent bead tracking. We have achieved 2-5 nm accuracy with a 2-50 ms time resolution.

Three-dimensional particle tracking via bifocal imaging. Publishing Authors By Initials

E Toprak,H Balci,BH Blehm,PR Selvin,

For similar equipment and supplies: microspheres research abstracts see: equipment and supplies: microspheres research

PUBMED ID PMID:

MEDLINE DATE:

Three-dimensional particle tracking via bifocal imaging. Journal Published:

PUBLICATION TYPE: Review

Journal: Nano letters

VOLUME: 7

Page Numbers: 2043-5

Journal Abbreviation: Nano Lett.

ISSN: 1530-6984

DAY: 21

MONTH: 06

YEAR: 2007

Three-dimensional particle tracking via bifocal imaging. Information

Number of References: 15

LANGUAGE: eng

NlmUniqueID: 101088070

Three-dimensional particle tracking via bifocal imaging. Keywords Mesh Terms:

KEYWORDS: Microspheres

MESH TERMS:

Chemical & Substance for Abstract: Three-dimensional particle tracking via bifocal imaging. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Three-dimensional particle tracking via bifocal imaging.

AFFILIATION: Department of Biophysics, University of Illinois Urbana Champaign, Urbana, Illinois 61801, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM68625

ACRONYM: GM

MEDLINETA: Nano Lett

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Three-dimensional particle tracking via bifocal imaging Related Publications

• Extent of voltage sensor movement during gating of shaker K+ channels.
• Increased mortality from lung cancer and bronchiectasis in young adults after exposure to arsenic in utero and in early childhood.
• New 9- or 10-dentate luminescent lanthanide chelates.
• New fluorescent tools for watching nanometer-scale conformational changes of single molecules.
• Single-molecule fluorescence to study molecular motors.
• The mitotic kinesin CENP-E is a processive transport motor.
• Three-dimensional particle tracking via bifocal imaging.
• Tracking melanosomes inside a cell to study molecular motors and their interaction.