Three-dimensional imaging of whole rodent organs using optical computed and emission tomography.

Three-dimensional imaging of whole rodent organs using optical computed and emission tomography. Research Abstract Details 

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Three-dimensional imaging of whole rodent organs using optical computed and emission tomography. Abstract Text:

Mark Oldham,Harshad Sakhalkar,Ying Min Wang,Pengyi Guo,Tim Oliver,Rex Bentley,Zeljko Vujaskovic,Mark Dewhirst,

We explore the potential of optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) in a new area-whole organ imaging. The techniques are implemented on an in-house prototype benchtop system with improved image quality and the capacity to image larger samples (up to 3 cm) than previous systems based on stereo microscopes. Imaging performance tests confirm high geometrical accuracy, accurate relative measurement of linear attenuation coefficients, and the ability to image features at the 50-microm level. Optical labeling of organ microvasculature was achieved using two stains deposited via natural in vivo circulatory processes: a passive absorbing ink-based stain and an active fluorescin FITC-lectin conjugate. The lectin protein binds to the endothelial lining, and FITC fluorescense enables optical-ECT imaging. Three-dimensional (3-D) optical-CT images have been acquired of a normal rat heart and left lung and a mouse right lung showing exquisite detail of the functional vasculature and relative perfusion distribution. Coregistered optical-ECT images were also acquired of the mouse lung and kidney. Histological sections confirmed effective labeling of microvasculature throughout the organs. The advantages of optical-CT and optical-ECT include the potential for a unique combination of high resolution and high contrast and compatibility with a wide variety of optical probes, including gene expression labeling fluorescent reporter proteins.

Three-dimensional imaging of whole rodent organs using optical computed and emission tomography. Publishing Authors By Initials

M Oldham,H Sakhalkar,YM Wang,P Guo,T Oliver,R Bentley,Z Vujaskovic,M Dewhirst,

For similar body regions: viscera research abstracts see: body regions: viscera research

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Three-dimensional imaging of whole rodent organs using optical computed and emission tomography. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of biomedical optics

VOLUME: 12

Page Numbers: 014009

Journal Abbreviation:

ISSN: 1083-3668

DAY: 3

MONTH: 12

YEAR: 2007

Three-dimensional imaging of whole rodent organs using optical computed and emission tomography. Information

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LANGUAGE: eng

NlmUniqueID: 9605853

Three-dimensional imaging of whole rodent organs using optical computed and emission tomography. Keywords Mesh Terms:

KEYWORDS: Viscera

MESH TERMS: radionuclide imaging

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Grant and Affiliation Information for Three-dimensional imaging of whole rodent organs using optical computed and emission tomography.

AFFILIATION: Duke University Medical Center, Department of Radiation Oncology and Biomedical Engineering, Durham, North Carolina 27710, USA. mark.oldham@duke.edu

Country: United States

AGENCY: United States NCI

GRANT: R01CA100835-02

ACRONYM: CA

MEDLINETA: J Biomed Opt

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