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Three-dimensional architecture of virus-packed tubule.

Three-dimensional architecture of virus-packed tubule. Research Abstract Details 

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  • Three-dimensional architecture of virus-packed tubule. Abstract Text:

    sumie katayamaSumie Katayama,taiyun weiTaiyun Wei,toshihiro omuraToshihiro Omura,junichi takagiJunichi Takagi,kenji iwasakiKenji Iwasaki,sumie katayamaSumie Katayama,taiyun weiTaiyun Wei,toshihiro omuraToshihiro Omura,junichi takagiJunichi Takagi,kenji iwasakiKenji Iwasaki,

    When rice dwarf virus (RDV), a member of the Reoviridae family, infects leafhopper cells, formation of protruding tubules composed of nonstructural viral protein Pns 10 can be observed. We examined the three-dimensional (3D) structure of these tubules containing RDV particles using electron tomography. The thin section of RDV-infected leafhopper vector cells in monolayers was subjected to double-tilt tomography. The tomographic 3D map provides a more reliable estimation of the real dimensions of the structure compared with the 2D image of the thin section. Docking of particle models made from atomic coordinates of RDV into the tomogram revealed that the inner diameter of the tubule was close to the outer diameter of the RDV particle. Fourier-transform of the reconstituted tubule image from the purified Pns 10 protein in vitro revealed a helical structure of the tubule.

    Three-dimensional architecture of virus-packed tubule. Publishing Authors By Initials

    s katayamaS Katayama,t weiT Wei,t omuraT Omura,j takagiJ Takagi,k iwasakiK Iwasaki,s katayamaS Katayama,t weiT Wei,t omuraT Omura,j takagiJ Takagi,k iwasakiK Iwasaki,

    For similar abstracts research abstracts see: abstracts research

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    Three-dimensional architecture of virus-packed tubule. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Journal of electron microscopy

    VOLUME: 56

    Page Numbers: 77-81

    Journal Abbreviation:

    ISSN: 0022-0744

    DAY: 30

    MONTH: Jun

    YEAR: 2007

    Three-dimensional architecture of virus-packed tubule. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 7611157

    Three-dimensional architecture of virus-packed tubule. Keywords Mesh Terms:

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    Grant and Affiliation Information for Three-dimensional architecture of virus-packed tubule.

    AFFILIATION: Institute for Protein Research, Laboratory of Protein Synthesis and Expression, Osaka University, 3-2 Yamada, Suita, Osaka 565-0871, Japan.

    Country: Japan

    Japan Research PublicationJapan Research Publication

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    MEDLINETA: J Electron Microsc (Tokyo)

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