The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins.

The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins. Research Abstract Details 

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The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins. Abstract Text:

The effect of chaotropic salts on the dissociation of ribonucleic acid from yeast nucleoprotein complex was studied. The effectiveness of various salts on the dissociation of ribonucleic acid followed the chaotropic series; i.e., Cl(3)CCOONa = NaClO(4) > NaBr > NaCl. Treatment of the nucleoprotein complex with 0.5M Cl(3)CCOONa or NaClO(4) resulted in RNA removal of about 80%, whereas NaCl and NaBr removed only about 10 and 25%, respectively. Based on the results presented, a simple and novel method for industrial-scale preparation of single-cell proteins with low levels of nucleic acid is proposed.

The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins. Publishing Authors By Initials

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The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology and bioengineering

VOLUME: 25

Page Numbers: 761-70

Journal Abbreviation: Biotechnol. Bioeng.

ISSN: 0006-3592

DAY: 12

MONTH: Mar

YEAR: 1983

The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins. Information

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LANGUAGE: eng

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Grant and Affiliation Information for The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins.

AFFILIATION: Institute of Food Science, Stocking Hall, Cornell University, Ithaca, New York 14853.

Country: United States

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MEDLINETA: Biotechnol Bioeng

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