The use of bright-field microscopy in evaluating bovine acrosome reaction.

The use of bright-field microscopy in evaluating bovine acrosome reaction. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The use of bright-field microscopy in evaluating bovine acrosome reaction. Abstract Text:

P Christensen,C H Whitfield,T J Parkinson,

A novel stain for evaluating the acrosomes of bovine spermatozoa was investigated. Acrosome reactions were induced by incubating spermatozoa enriched by swim-up with 1 mumol/l calcium ionophore A23187 for 1 or 1.5 h, while control samples were incubated in modified Tyrode's medium alone. After fixing in formaldehyde, spermatozoa were stained either with naphthol yellow S plus erythrosin B (NE) or with the novel stain, naphthol yellow S plus aniline blue (NA). The number of spermatozoa that had undergone acrosome reaction was counted and compared with results obtained using differential interference contrast microscopy (DIC). The correlation between the 2 staining methods was high (r=0.99), as was the correlation between NA staining and DIC (r=0.97). Slides stained with NA showed little background staining and the preparations were permanent. The results indicate that NA may be a useful stain for the bright-field evaluation of the bovine acrosome.

The use of bright-field microscopy in evaluating bovine acrosome reaction. Publishing Authors By Initials

P Christensen,CH Whitfield,TJ Parkinson,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The use of bright-field microscopy in evaluating bovine acrosome reaction. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 42

Page Numbers: 655-62

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Sep

YEAR: 1994

The use of bright-field microscopy in evaluating bovine acrosome reaction. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 421510

The use of bright-field microscopy in evaluating bovine acrosome reaction. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The use of bright-field microscopy in evaluating bovine acrosome reaction. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The use of bright-field microscopy in evaluating bovine acrosome reaction.

AFFILIATION: Hampshire Cattle Breeders Society Ltd. Beechen Lane, Lyndhurst, Hampshire, SO43 7NN, UK.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Theriogenology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The use of bright-field microscopy in evaluating bovine acrosome reaction Related Publications

• A "bucket of light" for viewing bacterial colonies in soft agar.
• A Geiger-Mueller counting unit and external quenching equipment for the estimation of C14 in carbon dioxide.
• A path forward for graphic designers in the NHS.
• Alaska sentinel surveillance for antimicrobial resistance in Helicobacter pylori isolates from Alaska native persons, 1999-2003.
• Ancient chemoreceptors retain their flexibility.
• Bacterial chemoreceptors: high-performance signaling in networked arrays.
• Behavioural trade-offs between growth and mortality explain evolution of submaximal growth rates.
• Closed suction drains do not increase the blood transfusion rates in patients undergoing total knee arthroplasty.
• Comparison of scars and resulting hypoaesthesia between the medial parapatellar and midline skin incisions in total knee arthroplasty.
• Cryptorchidism in North Ronaldsay sheep.
• Crystallographic studies of quadruplex nucleic acids.
• Dynamics of Helicobacter pylori-specific immunoglobulin G for 2 years after successful eradication of Helicobacter pylori infection in an American Indian and Alaska Native population.
• Eosinophilic xanthomatous granuloma with honeycomb lungs.
• Gradient of deficit in cone responses in the incomplete form of congenital stationary night blindness revealed by multifocal electroretinography.
• HLA associations in the antitumor response against malignant melanoma.
• Honeycomb lungs.
• I'LL BE JUDGE, I'LL BE JURY. REQUIREMENTS OF ADMINISTRATIVE DUE PROCESS IN DISCIPLINE OR EXPULSION OF MEMBERS OF A DENTAL SOCIETY.
• In vivo crosslinking methods for analyzing the assembly and architecture of chemoreceptor arrays.
• Lipoxin A4 stable analogs reduce allergic airway responses via mechanisms distinct from CysLT1 receptor antagonism.
• Naphtho[2,3-a]pyrene forms chiral domains on Au111.
• Phenotypic suppression methods for analyzing intra- and inter-molecular signaling interactions of chemoreceptors.
• Re: use of cyclo-oxygenase 2 inhibitors (COX-2) and prescription non-steroidal anti-inflammatory drugs (NSAIDS) in UK and USA populations: implications for COX-2 cardiovascular profile.
• Regenerator dependent photoinduced desorption of a dicarboxylated cyanine dye from the surface of single-crystal rutile.
• Relationship between fertility of bovine semen and In vitro induction of acrosome reactions by heparin.
• Response to: validation studies of the health improvement network (THIN) database for pharmacoepidemiology research by Lewis et al.
• The International Polar year 2007-2008; the Arctic human health legacy.
• The radiology of rheumatic heart disease.
• The radiology of rheumatic heart disease.
• The use of bright-field microscopy in evaluating bovine acrosome reaction.
• Ubiquitous pentacene monolayer on metals deposited onto pentacene films.