The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor.

The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor. Research Abstract Details 

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The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor. Abstract Text:

David A Widdick,Kieran Dilks,Govind Chandra,Andrew Bottrill,Mike Naldrett,Mechthild ,Tracy Palmer,

The twin-arginine translocation (Tat) pathway is a protein transport system for the export of folded proteins. Substrate proteins are targeted to the Tat translocase by N-terminal signal peptides harboring a distinctive R-R-x-Phi-Phi "twin-arginine" amino acid motif. Using a combination of proteomic techniques, the protein contents from the cell wall of the model Gram-positive bacterium Streptomyces coelicolor were identified and compared with that of mutant strains defective in Tat transport. The proteomic experiments pointed to 43 potentially Tat-dependent extracellular proteins. Of these, 25 were verified as bearing bona fide Tat-targeting signal peptides after independent screening with a facile, rapid, and sensitive reporter assay. The identified Tat substrates, among others, include polymer-degrading enzymes, phosphatases, and binding proteins as well as enzymes involved in secondary metabolism. Moreover, in addition to predicted extracellular substrates, putative lipoproteins were shown to be Tat-dependent. This work provides strong experimental evidence that the Tat system is used as a major general export pathway in Streptomyces.

The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor. Publishing Authors By Initials

DA Widdick,K Dilks,G Chandra,A Bottrill,M Naldrett,M ,T Palmer,

For similar bacteria: endospore-forming bacteria: gram-positive endospore-forming bacteria: gram-positive endospore-forming rods: streptomycetaceae: streptomyces: streptomyces coelicolor research abstracts see: bacteria: endospore-forming bacteria: gram-positive endospore-forming bacteria: gram-positive endospore-forming rods: streptomycetaceae: streptomyces: streptomyces coelicolor research

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The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 17927-32

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 8

MONTH: 11

YEAR: 2006

The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor. Keywords Mesh Terms:

KEYWORDS: Streptomyces coelicolor

MESH TERMS: metabolism

Chemical & Substance for Abstract: The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor. Information

Substance Name: agarase

Registry Number: EC 3.2.1.81

Grant and Affiliation Information for The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor.

AFFILIATION: Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, United Kingdom.

Country: United States

AGENCY: United States NIGMS

GRANT: T32-GM07229

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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