The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain.

The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain. Research Abstract Details 

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The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain. Abstract Text:

HNF1 and C/EBP alpha are transcription factors that bind to and trans-activate the human albumin gene proximal promoter. Various 5' deletions of the human albumin promoter were coupled to a luciferase reporter gene (alb-luc constructs) and co-electroporated with HNF1 and/or C/EBP alpha expression vectors into HeLa cells. Luciferase activities from co-electroporation of the HNF1 and C/EBP alpha expression vectors with the alb-luc constructs were approximately 10-fold greater than the sum of the activities achieved with HNF1 and C/EBP alpha alone. Analysis of COOH-terminal or internal deletions of the HNF1 expression vector revealed that the domain important for collaborative interaction with C/EBP alpha could be localized to a 157 amino acid region not previously described. This domain is proline and glutamine-rich and is highly homologous (66%) to a portion of vHNF1, an evolutionarily related gene first identified in dedifferentiated hepatoma cells. A construct linking the negatively charged activation domain of herpes simplex virus protein VP16 to the DNA-binding domain of HNF1 showed that it could also synergize with C/EBP alpha to trans-activate the human albumin gene promoter. Our studies delineate a domain in HNF1 important for synergistic activation with C/EBP alpha.

The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain. Publishing Authors By Initials

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 269

Page Numbers: 1177-82

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 14

MONTH: Jan

YEAR: 1994

The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain. Information

Substance Name: Hepatocyte Nuclear Factor 1-beta

Registry Number: 138674-15-4

Grant and Affiliation Information for The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain.

AFFILIATION: Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.

Country: UNITED STATES

AGENCY: United States NIGMS

GRANT: GM32111

ACRONYM: GM

MEDLINETA: J Biol Chem

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