The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes.

The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes. Abstract Text:

Bong Kim,Alexey I Nesvizhskii,P Geetha Rani,Steven Hahn,Ruedi Aebersold,Jeffrey A Ranish,

In this article, we provide direct evidence that the evolutionarily conserved transcription elongation factor TFIIS functions during preinitiation complex assembly. First, we identified TFIIS in a mass spectrometric screen of RNA polymerase II (Pol II) preinitiation complexes (PICs). Second, we show that the association of TFIIS with a promoter depends on functional PIC components including Mediator and the SAGA complex. Third, we demonstrate that TFIIS is required for efficient formation of active PICs. Using truncation mutants of TFIIS, we find that the Pol II-binding domain is the minimal domain necessary to stimulate PIC assembly. However, efficient formation of active PICs requires both the Pol II-binding domain and the poorly understood N-terminal domain. Importantly, Domain III, which is required for the elongation function of TFIIS, is dispensable during PIC assembly. The results demonstrate that TFIIS is a PIC component that is required for efficient formation and/or stability of the complex.

The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes. Publishing Authors By Initials

B Kim,AI Nesvizhskii,PG Rani,S Hahn,R Aebersold,JA Ranish,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 16068-73

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 3

MONTH: 10

YEAR: 2007

The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes.

AFFILIATION: Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: PM50GM076547

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes Related Publications

• Metabolic dependence of fast axoplasmic transport in nerve.
• The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes.