The time resolved fluorescence and anisotropy of subtilisins BPN' and Carlsberg.

The time resolved fluorescence and anisotropy of subtilisins BPN' and Carlsberg. Research Abstract Details 

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The time resolved fluorescence and anisotropy of subtilisins BPN' and Carlsberg. Abstract Text:

J M Janot,A Beeby,P M Bayley,D Phillips,

Time-resolved emission and anisotropy have been measured for the tryptophan (Trp) residues of two closely related subtilisin proteins. The single Trp of subtilisin Carlsberg shows complex lifetime properties, and anisotropy consistent with a fast (ca. 200 ps) segmental motion, on the "wobbling in a cone model" the semi angle is in the range 38 to 47 degrees. The lifetime and anisotropy properties for this single Trp residue suggest that the predominant state is that of an effectively non-emitting statically quenched fluorophore. This fast component is also resolved in the anisotropy of subtilisin BPN' but with relatively low amplitude, due to the dominant emission of the other Trp residues. The diversity of the photophysical properties is not readily correlated with the structure of the proteins, though the observed complexity is consistent with the likely heterogeneity of environment due to the surface location of all the Trp residues.

The time resolved fluorescence and anisotropy of subtilisins BPN' and Carlsberg. Publishing Authors By Initials

JM Janot,A Beeby,PM Bayley,D Phillips,

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The time resolved fluorescence and anisotropy of subtilisins BPN' and Carlsberg. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biophysical chemistry

VOLUME: 41

Page Numbers: 277-87

Journal Abbreviation: Biophys. Chem.

ISSN: 0301-4622

DAY: 3

MONTH: Dec

YEAR: 1991

The time resolved fluorescence and anisotropy of subtilisins BPN' and Carlsberg. Information

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LANGUAGE: eng

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AFFILIATION: Laboratoire de Physico-Chimie des Systemes Polyphases, C.N.R.S route de Mende BP5051, 34033 Montpellier Cedex France.

Country: Netherlands

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MEDLINETA: Biophys Chem

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