The teleost fish medaka (Oryzias latipes) as genetic model to study gravity dependent bone homeostasis in vivo.

The teleost fish medaka (Oryzias latipes) as genetic model to study gravity dependent bone homeostasis in vivo. Research Abstract Details 

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The teleost fish medaka (Oryzias latipes) as genetic model to study gravity dependent bone homeostasis in vivo. Abstract Text:

T U Wagner,J Renn,T Riemensperger,J-N Volff,R W Koster,R Goerlich,M Schartl,C Winkler,

Long-term space flight and microgravity result in bone loss that can be explained by reduced activity of bone-forming cells (osteoblasts) and/or an increase in activity of bone resorbing cells (osteoclasts). Osteoprotegerin (OPG) has been shown to regulate the balance between osteoblast and osteoclast cell numbers and is involved in maintaining constant bone mass under normal gravitational conditions. The small bony fish medaka (Oryzias latipes) has attracted increasing attention as a genetic model system to study normal embryonic developmental and pathological processes. To analyze the molecular mechanisms of bone formation in this small vertebrate, we have isolated two opg genes, opgl and opg2, from medaka. Our phylogenetic analysis reveals that both genes originated from a common ancestor by fish-specific gene duplication and represent the orthologs of the mammalian opg gene. Both opg genes are differentially expressed during embryonic and larval development, in adult tissues and in cultured primary osteoblast-like cells. Furthermore, we have characterized the opg2 promoter region and identified consensus binding sites for the transcription factor core-binding-factor-1A (CBFA1). In mammals, CBFA1 has been shown to be a regulator of opg expression and to be essential for several steps during osteoblast differentiation. Here we show that sequence and expression domains of opg, cbfal and a member of the dlx gene family are highly conserved between medaka and higher vertebrates. This suggests that not only single genes but entire genetic networks for bone formation are conserved between teleosts and mammals. These findings will open medaka fish as a genetic model to monitor bone formation under different gravity conditions in a living whole animal allowing the identification of novel factors involved in bone homeostasis.

The teleost fish medaka (Oryzias latipes) as genetic model to study gravity dependent bone homeostasis in vivo. Publishing Authors By Initials

TU Wagner,J Renn,T Riemensperger,JN Volff,RW Koster,R Goerlich,M Schartl,C Winkler,

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The teleost fish medaka (Oryzias latipes) as genetic model to study gravity dependent bone homeostasis in vivo. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Advances in space research : the official journal

VOLUME: 32

Page Numbers: 1459-65

Journal Abbreviation: Adv Space Res

ISSN: 0273-1177

DAY: 4

MONTH: 03

YEAR: 2003

The teleost fish medaka (Oryzias latipes) as genetic model to study gravity dependent bone homeostasis in vivo. Information

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LANGUAGE: eng

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AFFILIATION: Univ. of Wurzburg, Dept. of Physiological Chemistry I, Wurzburg, Germany.

Country: United States

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MEDLINETA: Adv Space Res

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