The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation.

The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation. Abstract Text:

Shiro Suzuki,Masaomi Yamamura,Takefumi Hattori,Tomoyuki Nakatsubo,Toshiaki Umezawa,Shiro Suzuki,Masaomi Yamamura,Takefumi Hattori,Tomoyuki Nakatsubo,Toshiaki Umezawa,Shiro Suzuki,Masaomi Yamamura,Takefumi Hattori,Tomoyuki Nakatsubo,Toshiaki Umezawa,

The selective formation of E- or Z-isomers is an important process in natural product metabolism. We show that the subunit composition of an enzyme can alter the geometrical composition of the enzymatic products. Hinokiresinol synthase, purified from Asparagus officinalis cell cultures, is responsible for the conversion of (7E,7'E)-4-coumaryl 4-coumarate to (Z)-hinokiresinol, the first step in norlignan formation. The protein is most likely a heterodimer composed of two distinct subunits, which share identity with members of the phloem protein 2 gene superfamily. Interestingly, each recombinant subunit of hinokiresinol synthase expressed in Escherichia coli solely converted (7E,7'E)-4-coumaryl 4-coumarate to the unnatural (E)-hinokiresinol, the E-isomer of (Z)-hinokiresinol. By contrast, a mixture of recombinant subunits catalyzed the formation of (Z)-hinokiresinol from the same substrate.

The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation. Publishing Authors By Initials

S Suzuki,M Yamamura,T Hattori,T Nakatsubo,T Umezawa,S Suzuki,M Yamamura,T Hattori,T Nakatsubo,T Umezawa,S Suzuki,M Yamamura,T Hattori,T Nakatsubo,T Umezawa,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 21008-13

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 1091-6490

DAY: 19

MONTH: 12

YEAR: 2007

The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation.

AFFILIATION: Research Institute for Sustainable Humanosphere and Institute of Sustainability Science, Kyoto University, Uji, Kyoto 611-0011, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation Related Publications

• A genetic method to identify mitochondrial proteins in living Mammalian cells.
• Bleomycin and other antitumor antibiotics of high molecular weight.
• Current status of Japanese studies with the new anthracycline antibiotic aclacinomycin A.
• Fundamental studies on bestatin: a small molecular microbial product enhancing immune response.
• Hyaline cartilage formation and enchondral ossification modeled with KUM5 and OP9 chondroblasts.
• Identification of proteins targeted into the endoplasmic reticulum by cDNA library screening.
• Inhibition of CDP-DG: inositol transferase by inostamycin.
• Inhibition of G1 cyclin expression in normal rat kidney cells by inostamycin, a phosphatidylinositol synthesis inhibitor.
• Inhibition of nucleic acid biosynthesis in cell-free systems of Escherichia coli B by pluramycin.
• Inhibition of protein synthesis by bottromycin A2 and its hydrazide.
• Kinetic studies on the inhibition of nucleoside diphosphate kinase by desdanine.
• Local-density approximation for orbital densities applied to the self-interaction correction.
• Mechanism of kasugamycin action on polypeptide synthesis.
• Messenger RNA-associated 30S ribosomal subunit: extraction from E. coli and the effect of chloramphenicol on the content.
• Mode of action of enomycin, an antitumor antibiotic of high molecular weight. 3. On the weak inhibition of polyphenylalanine synthesis directed by polyuridylic acid.
• Mode of action of enomycin, an antitumor antibiotic of high molecular weight. I. Inhibition of protein synthesis.
• Mode of action of enomycin, an antitumor antibiotic of high molecular weight. II. Preferential binding of enomycin to mammalian ribosomes.
• NF-kappaB inhibitor dehydroxymethylepoxyquinomicin suppresses osteoclastogenesis and expression of NFATc1 in mouse arthritis without affecting expression of RANKL, osteoprotegerin or macrophage colony-stimulating factor.
• Natural and artificial bleomycins: chemistry and antitumor activities.
• New anthracycline antibiotic aclacinomycin A: experimental studies and correlations with clinical trials.
• New microbial secondary metabolites under preclinical development for cancer treatment.
• Optical probes to identify the glucocorticoid receptor ligands in living cells.
• Pyruvate kinase of Escherichia coli. Its role in supplying nucleoside triphosphates in cells under anaerobic conditions.
• Screening of small molecular microbial products modulating immune responses and bestatin.
• Studies on antitumor substances of microbial origin.
• The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation.
• Ways for a mesenchymal stem cell to live on its own: maintaining an undifferentiated state ex vivo.
• [Method for determination of the bleomycin inactivating enzyme acitivity in tissues]
• [Screening of bioactive metabolites that suppress inflammation]
• [Uptake and translocation of 14C-labelled Kasugamycin in rice plants]