The subtypes of nicotinic acetylcholine receptors on dopaminergic terminals of mouse striatum.

The subtypes of nicotinic acetylcholine receptors on dopaminergic terminals of mouse striatum. Research Abstract Details 

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The subtypes of nicotinic acetylcholine receptors on dopaminergic terminals of mouse striatum. Abstract Text:

Sharon R Grady,Outi Salminen,Duncan C Laverty,Paul Whiteaker,J Michael McIntosh,Allan C Collins,Michael J Marks,

This review summarizes studies that attempted to determine the subtypes of nicotinic acetylcholine receptors (nAChR) expressed in the dopaminergic nerve terminals in the mouse. A variety of experimental approaches has been necessary to reach current knowledge of these subtypes, including in situ hybridization, agonist and antagonist binding, function measured by neurotransmitter release from synaptosomal preparations, and immunoprecipitation by selective antibodies. Early developments that facilitated this effort include the radioactive labeling of selective binding agents, such as [(125)I]-alpha-bungarotoxin and [(3)H]-nicotine, advances in cloning the subunits, and expression and evaluation of function of combinations of subunits in Xenopus oocytes. The discovery of epibatidine and alpha-conotoxin MII (alpha-CtxMII), and the development of nAChR subunit null mutant mice have been invaluable in determining which nAChR subunits are important for expression and function in mice, as well as allowing validation of the specificity of subunit specific antibodies. These approaches have identified five nAChR subtypes of nAChR that are expressed on dopaminergic nerve terminals. Three of these contain the alpha6 subunit (alpha4alpha6beta2beta3, alpha6beta2beta3, alpha6beta2) and bind alpha-CtxMII with high affinity. One of these three subtypes (alpha4alpha6beta2beta3) also has the highest sensitivity to nicotine of any native nAChR that has been studied, to date. The two subtypes that do not have high affinity for alpha-CtxMII (alpha4beta2, alpha4alpha5beta2) are somewhat more numerous than the alpha6* subtypes, but do bind nicotine with high affinity. Given that our first studies detected readily measured differences in sensitivity to agonists and antagonists among these five nAChR subtypes, it seems likely that subtype selective compounds could be developed that would allow therapeutic manipulation of diverse nAChRs that have been implicated in a number of human conditions.

The subtypes of nicotinic acetylcholine receptors on dopaminergic terminals of mouse striatum. Publishing Authors By Initials

SR Grady,O Salminen,DC Laverty,P Whiteaker,JM McIntosh,AC Collins,MJ Marks,

For similar proteins: membrane proteins: receptors, cell surface: receptors, neurotransmitter: receptors, cholinergic: receptors, nicotinic research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, neurotransmitter: receptors, cholinergic: receptors, nicotinic research

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The subtypes of nicotinic acetylcholine receptors on dopaminergic terminals of mouse striatum. Journal Published:

PUBLICATION TYPE: Review

Journal: Biochemical pharmacology

VOLUME: 74

Page Numbers: 1235-46

Journal Abbreviation: Biochem. Pharmacol.

ISSN: 0006-2952

DAY: 27

MONTH: 07

YEAR: 2007

The subtypes of nicotinic acetylcholine receptors on dopaminergic terminals of mouse striatum. Information

Number of References: 119

LANGUAGE: eng

NlmUniqueID: 101032

The subtypes of nicotinic acetylcholine receptors on dopaminergic terminals of mouse striatum. Keywords Mesh Terms:

KEYWORDS: Receptors, Nicotinic

MESH TERMS: classification

Chemical & Substance for Abstract: The subtypes of nicotinic acetylcholine receptors on dopaminergic terminals of mouse striatum. Information

Substance Name: Dopamine

Registry Number: 51-61-6

Grant and Affiliation Information for The subtypes of nicotinic acetylcholine receptors on dopaminergic terminals of mouse striatum.

AFFILIATION: Institute for Behavioral Genetics, University of Colorado, Boulder, CO 80309, USA. sharon.grady@colorado.edu

Country: England

AGENCY: United States NIDA

GRANT: DA12242

ACRONYM: DA

MEDLINETA: Biochem Pharmacol

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