The structure of Escherichia coli signal recognition particle revealed by scanning transmission electron microscopy.

The structure of Escherichia coli signal recognition particle revealed by scanning transmission electron microscopy. Research Abstract Details 

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The structure of Escherichia coli signal recognition particle revealed by scanning transmission electron microscopy. Abstract Text:

Iain L Mainprize,Daniel R Beniac,Elena Falkovskaia,Robert M Cleverley,Lila M Gierasch,F Peter Ottensmeyer,David W Andrews,

Structural studies on various domains of the ribonucleoprotein signal recognition particle (SRP) have not converged on a single complete structure of bacterial SRP consistent with the biochemistry of the particle. We obtained a three-dimensional structure for Escherichia coli SRP by cryoscanning transmission electron microscopy and mapped the internal RNA by electron spectroscopic imaging. Crystallographic data were fit into the SRP reconstruction, and although the resulting model differed from previous models, they could be rationalized by movement through an interdomain linker of Ffh, the protein component of SRP. Fluorescence resonance energy transfer experiments determined interdomain distances that were consistent with our model of SRP. Docking our model onto the bacterial ribosome suggests a mechanism for signal recognition involving interdomain movement of Ffh into and out of the nascent chain exit site and suggests how SRP could interact and/or compete with the ribosome-bound chaperone, trigger factor, for a nascent chain during translation.

The structure of Escherichia coli signal recognition particle revealed by scanning transmission electron microscopy. Publishing Authors By Initials

IL Mainprize,DR Beniac,E Falkovskaia,RM Cleverley,LM Gierasch,FP Ottensmeyer,DW Andrews,

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The structure of Escherichia coli signal recognition particle revealed by scanning transmission electron microscopy. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular biology of the cell

VOLUME: 17

Page Numbers: 5063-74

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 20

MONTH: 09

YEAR: 2006

The structure of Escherichia coli signal recognition particle revealed by scanning transmission electron microscopy. Information

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LANGUAGE: eng

NlmUniqueID: 9201390

The structure of Escherichia coli signal recognition particle revealed by scanning transmission electron microscopy. Keywords Mesh Terms:

KEYWORDS: Solutions

MESH TERMS: ultrastructure

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Substance Name: Solutions

Registry Number: 0

Grant and Affiliation Information for The structure of Escherichia coli signal recognition particle revealed by scanning transmission electron microscopy.

AFFILIATION: Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton L8N 3Z5, Canada.

Country: United States

AGENCY: United States NIGMS

GRANT: GM-34962

ACRONYM: GM

MEDLINETA: Mol Biol Cell

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