The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase.

The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. Abstract Text:

K Watanabe,

Mild alkaline treatment of glycopeptide (GP-II) resulted in the loss of 1 mole of serine and 5 moles of threonine per mole of GP-II, suggesting the presence of O-glycosyl bonds between 1 serine and 5 threonine residues and carbohydrate chains. Treatment of GP-II with alkaline borohydride released only disaccharide. Methylation studies of the carbohydrate moiety gave 2,3,4,6-tetra-O-methyl and 2,4,6-tri-O-methyl derivatives of mannose in a ratio of approximately 1:1. In addition, one step of Smith degradation resulted in the loss of about 6 residues of mannose per mole of GP-II. Moreover, alpha-mannosidase [EC 3.2.1.24] liberated about 6 residles of mannose per mole of GP-II. On the basis of these data, the structure of the carbohydrate moiety of GP-II was confirmed to be 3-O-alpha-mannosylmannose. The amino- and carboxyl-terminal amino acids of GP-II were determined to be threonine and serine, respectively. On reductive cleavage of N-proline bonds with metallic sodium in liquid ammonia, 2 moles of alanine per mole of GP-II were lost. From the compositions of three fragments isolated from the reductive cleavage products, the amino acid sequence of the peptide portion of GP-II was determined. Based on these data, a probable structure was proposed for GP-II.

The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. Publishing Authors By Initials

K Watanabe,

For similar fungi: zygomycota: mucorales: rhizopus research abstracts see: fungi: zygomycota: mucorales: rhizopus research

PUBMED ID PMID:

MEDLINE DATE:

The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 80

Page Numbers: 379-87

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1976

The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. Keywords Mesh Terms:

KEYWORDS: Rhizopus

MESH TERMS: enzymology

Chemical & Substance for Abstract: The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. Information

Substance Name: Glycoside Hydrolases

Registry Number: EC 3.2.1.-

Grant and Affiliation Information for The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The structure of a glycopeptide GP-II isolated from Rhizopus saccharogenic amylase Related Publications

• Analysis of the CC chemokine receptor 5 delta32 polymorphism in a Brazilian population with cutaneous leishmaniasis.
• Behavioral Determination of Odor Preference is Coded by the Oscillation Frequency in a Collective Oscillating Network of a Terrestrial Mollusk.
• Cost-effectiveness of radiofrequency ablation and surgical therapy for small hepatocellular carcinoma of 3cm or less in diameter.
• Decreased renal expressions of heat shock protein-72 and -25 are associated with renal dysfunction in biliary cirrhotic rats.
• Design of a synchrotron radiation source at Tohoku University.
• Divergent activities of interferon-alpha subtypes against intracellular hepatitis C virus replication.
• Effect of the moderate exercise on performance in upper/lower limb during long term bed rest.
• Effects of aminoglutethimide phosphate on litter size and birth weight in superovulated rats.
• Efficacy of ecabet sodium enema on steroid resistant or steroid dependent ulcerative colitis.
• Efficient Gene Transfer into Differentiated Human Trophoblast Cells with Adenovirus Vector Containing RGD Motif in the Fiber Protein.
• Enhancement of cytoprotective ability and cell restoration in 70-kDa heat shock protein gene-transfected rat gastric mucosal cells.
• Epimorphin expression and stellate cell status in mouse liver injury.
• Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice.
• Genipin prevents fulminant hepatic failure resulting in reduction of lethality through the suppression of TNF-alpha production.
• Heart rate turbulence slope reduction in imminent ventricular tachyarrhythmia and its implications.
• Hepatic gene expression in hepatocyte-specific Pten deficient mice showing steatohepatitis without ethanol challenge.
• High-performance EUV/soft X-ray ellipsometry system using multilayer mirrors.
• Hydrodynamics-based gene delivery of naked DNA encoding fetal liver kinase-1 gene effectively suppresses the growth of pre-existing tumors.
• Mucins and ocular surface disease.
• Optical design for a bending-magnet beamline based on a varied-line-spacing plane grating.
• Proline promoted synthesis of ring-fused homodimers: self-condensation of alpha,beta-unsaturated aldehydes.
• Pyocyanin isolated from a marine microbial population: synergistic production between two distinct bacterial species and mode of action.
• Root caries prevalence in a group of Brazilian adult dental patients.
• Scrotal imaging.
• Synthesis and biological activity of 5',9-anhydro-3-purine-isonucleosides as potential anti-hepatitis C virus agents.
• Technical note: measurement of ferritin in bovine milk and its clinical significance.
• Transmission multilayer polarizers for use in the 55-90 eV region.
• Treatment for intercostal arterial aneurysm in neurofibromatosis type 1.
• Two cases requiring external reinforcement in perceptual learning.
• Well-differentiated adenocarcinoma mimicking complete-type intestinal metaplasia in the stomach.