The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata.

The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata. Abstract Text:

Michio Suzuki,Hiromichi Nagasawa,Michio Suzuki,Hiromichi Nagasawa,

The mollusk shell is a hard tissue consisting of calcium carbonate and organic matrices. The organic matrices are considered to play important roles in shell formation. We have previously identified a prismatic layer-specific protein named Prismalin-14, which consists of 105 amino acid residues and includes four structurally characteristic regions; a repeated sequence of Pro-Ile-Tyr-Arg, a Gly/Tyr-rich region and N- and C-terminal Asp-rich regions. Prismalin-14 showed an inhibitory activity on calcium carbonate precipitation and a calcium-binding ability in vitro. In this study, we prepared some molecular species of recombinant proteins including Prismalin-14 and its truncated proteins in an Escherichia coli expression system to reveal a structure-function relationship of Prismalin-14. The results showed that the Gly/Tyr-rich region was responsible for chitin binding and was identified as a novel chitin-binding sequence. On the other hand, both N- and C-terminal Asp-rich regions are related to inhibitory activity on calcium carbonate precipitation in vitro. Immunohistological observation revealed that Prismalin-14 was localized at the acid-insoluble organic framework including chitin. All these results strongly suggest that Prismalin-14 is a framework protein that mediates chitin and calcium carbonate crystals by using its acidic and chitin-binding regions.

The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata. Publishing Authors By Initials

M Suzuki,H Nagasawa,M Suzuki,H Nagasawa,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The FEBS journal

VOLUME: 274

Page Numbers: 5158-66

Journal Abbreviation: FEBS J.

ISSN: 1742-464X

DAY: 5

MONTH: 09

YEAR: 2007

The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101229646

The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata.

AFFILIATION: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: FEBS J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata Related Publications

• A method to determine the affinity of heparin to thrombin and antithrombin III on equilibrium gel permeation chromatography.
• A novel paradigm for assessing efficacies of potential antidotes against neurotoxins in mice.
• All-solid-state high-power conduction-cooled Nd:YLF rod laser.
• Characterization of Nalpha-benzyloxycarbonyl-L-lysine oxidizing enzyme from Rhodococcus sp. AIU Z-35-1.
• Characterization of the interaction between human protein S and C4b-binding protein (C4bp).
• Detergent-resistant membrane domains are required for mast cell activation but dispensable for tyrosine phosphorylation upon aggregation of the high affinity receptor for IgE.
• Differences and relationships between residual volumes measured on land and in water calculated from estimate equations.
• Effect of ENA modification of triplex-forming oligonucleotide on pyrimidine motif triplex formation.
• Identification of chitin in the prismatic layer of the shell and a chitin synthase gene from the Japanese pearl oyster, Pinctada fucata.
• L-arginine administration reverses anemia associated with renal disease.
• Limited proteolysis of complement protein C3b by regulatory enzyme C3b inactivator: isolation and characterization of a biologically active fragment, C3d,g.
• Limited proteolysis of the third component of human complement, C3, by heat treatment.
• Mapping of the sites responsible for factor I-cofactor activity for cleavage of C3b and C4b on human C4b-binding protein (C4bp) by deletion mutagenesis.
• Molecular cloning and expression analysis of ecdysone receptor and retinoid X receptor from the kuruma prawn, Marsupenaeus japonicus.
• Organocatalytic asymmetric nitroaldol reaction: cooperative effects of guanidine and thiourea functional groups.
• Preparation of biologically active fluorescent heparin composed of fluorescein-labeled species and its behavior to antithrombin III.
• Purification and Characterization of a Dehydrogenase Catalyzing Conversion of N(alpha)-Benzyloxycarbonyl-L-Aminoadipic-delta-Semialdehyde to N(alpha)-Benzyloxycarbonyl-L-Aminoadipic Acid from Rhodococcus sp. AIU Z-35-1.
• Purification of carboxypeptidase A using Sepharose 4B-bound 3-phenylpropionate.
• Raman spectroscopic studies on the interaction between counterion and polyion.
• Some enzymatic properties of peptidyl dipeptide hydrolase (angiotensin I-converting enzyme).
• Stereospecificity of peptidyl dipeptide hydrolase (angiotensin I-converting enzyme).
• Studies on human high molecular weight (HMW) kininogen. II. Structural change of HMW kininogen by the action of human plasma kallikrein.
• Studies on human high molecular weight (HMW) kininogen. III. Cleavage of HMW kininogen by the action of human salivary kallikrein.
• Studies on human kininogens. I. Isolation, characterization, and cleavage by plasma kallikrein of high molecular weight (HMW)-kininogen.
• Studies on the interaction between alkali metal cations and polyion by sound velocity.
• Successful high-dose methotrexate chemotherapy in a patient with acute lymphocytic leukemia who developed acute renal failure during the initial treatment.
• Sulfated heteropolysaccharide-peptide complexes from oyster viscera.
• The chemokine CXCL12 and regulation of HSC and B lymphocyte development in the bone marrow niche.
• The structure-function relationship analysis of Prismalin-14 from the prismatic layer of the Japanese pearl oyster, Pinctada fucata.
• [Progress in study of glomerulonephritis]