The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions.

The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions. Abstract Text:

Using three lines of human normal glial cells and four established lines of human malignant glioma cells we have studied cell spreading following seeding onto glass and plastic substrata. The cells were detached with EDTA and trypsin, suspended in EMEM with 10% calf serum and studied with time-lapse, phase-contrast cinematography in suspension and during attachment and spreading. Cells were fixed and prepared for light microscopy while in suspension and during the spreading process. They were also prepared for scanning and transmission electron microscopy at different times during spreading. The projected areas of stained cells, in suspension and at different stages of spreading, were measured morphometrically and the results compared statistically. The glial cells in suspension were often found to retain somewhat their shape from the previous monolayer. They spread radially outwards with even lamellar cytoplasm and peripheral ruffling, as a group more quickly than the malignant glioma cells. They also became polarized and started to translocate in a shorter time. The glioma cells were spherical in suspension and characterized by pronounced blebbing of the cell surface. Blebbing continued during spreading and was finally replaced by ruffling at the edge. The cells spread like the glial cells radially outwards but the lamellar cytoplasm was occasionally somewhat irregular. Cells from the glioma lines spread as groups slower than the glial cells but with individual rates for the different lines. One of the glioma lines appeared to spread more thinly than the glial cells. Cells which sedimented on top of other cells could not spread. Aggregations of cells spread and became polarized more quickly than single cells in all cases.

The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions. Publishing Authors By Initials

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

PUBMED ID PMID:

MEDLINE DATE:

The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta pathologica, microbiologica, et immunologica

VOLUME: 93

Page Numbers: 235-49

Journal Abbreviation:

ISSN: 0108-0164

DAY: 26

MONTH: Sep

YEAR: 1985

The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8206622

The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions.

AFFILIATION:

Country: DENMARK

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Acta Pathol Microbiol Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The spreading of human normal glial and malignant glioma cells in culture Studies on standard culture conditions Related Publications

• Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I.
• Amyloid fibril composition is related to the phenotype of hereditary transthyretin V30M amyloidosis.
• Amyloid of human islets of Langerhans. II. Electron microscopic analysis of isolated amyloid.
• Amyloidogenic potential of foie gras.
• Amyloidosis in ageing obese-hyperglycemic mice and their lean litter-mates. A morphological study.
• An established human cell line derived from a malignant mediastinal teratoma.
• Density dependent cell cycle inhibition--a universal growth control among normal animal cells.
• Expression of the c-myc proto-oncogene in growth stimulated porcine thyroid follicle cells.
• Germ Line Origin and Somatic Mutations Determine the Target Tissues in Systemic AL-Amyloidosis.
• Growth factor receptors in thyroid follicle cells.
• Growth stimulation of aged cells in culture.
• In vitro differentiation and specialization of cell surfaces in human glia-like cells.
• Lactadherin binds to elastin--a starting point for medin amyloid formation?
• Occurrence of amyloid deposits in the skin in secondary systemic amyloidosis.
• PDGF-like growth factors in autocrine stimulation of growth.
• Plasma membrane motility of cultured human glia cells in phase II and III.
• Psychopathology and personality traits in patients with treated Wilson disease grouped according to gene mutations.
• Rapid induction of experimental AA amyloidosis in mink by intravenous injection of amyloid enhancing factor.
• Real-time monitoring of apoptosis by caspase-3-like protease induced FRET reduction triggered by amyloid aggregation.
• Role of aggregated medin in the pathogenesis of thoracic aortic aneurysm and dissection.
• Skin involvement in secondary amyloidosis.
• Structural and functional properties of thyroid follicle cells in culture.
• Studies on human foetal endocrine pancreas in vitro.
• Substance P and enteroglucagon-like immunoreactivity in argentaffin and argyrophil midgut carcinoid tumours.
• The fine structure of growing human glia and glioma cells. Whole cell preparations.
• The pancreatic islets in systemic amyloidosis. The occurrence of two different types of amyloid.
• The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions.
• Transthyretin and amyloid in the islets of langerhans in type-2 diabetes.
• Ultrastructural features of cultured human glia and glioma cells.
• Unreliability of immunohistochemistry for typing amyloid deposits.