The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes.

The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes. Research Abstract Details 

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The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes. Abstract Text:

The accuracy of chromosome segregation is enhanced by the spindle assembly checkpoint (SAC). The SAC is thought to monitor two distinct events: attachment of kinetochores to microtubules and the stretch of the centromere between the sister kinetochores that arises only when the chromosome becomes properly bioriented. We examined human cells undergoing mitosis with unreplicated genomes (MUG). Kinetochores in these cells are not paired, which implies that the centromere cannot be stretched; however, cells progress through mitosis. A SAC is present during MUG as cells arrest in response to nocodazole, taxol, or monastrol treatments. Mad2 is recruited to unattached MUG kinetochores and released upon their attachment. In contrast, BubR1 remains on attached kinetochores and exhibits a level of phosphorylation consistent with the inability of MUG spindles to establish normal levels of centromere tension. Thus, kinetochore attachment to microtubules is sufficient to satisfy the SAC even in the absence of interkinetochore tension.

The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes. Publishing Authors By Initials

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The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of cell biology

VOLUME: 183

Page Numbers: 29-36

Journal Abbreviation: J. Cell Biol.

ISSN: 1540-8140

DAY: 29

MONTH: 09

YEAR: 2008

The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes. Information

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LANGUAGE: eng

NlmUniqueID: 375356

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AFFILIATION: Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA. oconnell@wadsworth.org

Country: United States

AGENCY: United States NIGMS

GRANT: GM077911

ACRONYM: GM

MEDLINETA: J Cell Biol

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