The sites on calmodulin cross-linked to myosin light chain kinase and troponin I with water-soluble carbodiimide.

The sites on calmodulin cross-linked to myosin light chain kinase and troponin I with water-soluble carbodiimide. Research Abstract Details 

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The sites on calmodulin cross-linked to myosin light chain kinase and troponin I with water-soluble carbodiimide. Abstract Text:

K Yamamoto,T Sekine,K Sutoh,

To elucidate the interaction of calmodulin with calmodulin binding proteins, we studied the location of the interaction sites on calmodulin by using a chemical cross-linking reagent. Calmodulin prepared from wheat germ was cross-linked to myosin light chain kinase and troponin-I with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide. The cross-linked products were cleaved partially with cyanogen bromide and cross-linked sites were determined by peptide mapping analysis using SDS-urea polyacrylamide gel electrophoresis. Peptides which contain the cross-linked site were displaced from their position because of the attached fragments of myosin light chain kinase or troponin I. The peptide of calmodulin from the N-terminal to Met-73 in the cross-linked product with myosin light chain kinase had the same mobility as that of uncross-linked calmodulin on the map though the amount of the peptide was decreased in the cross-linked product. The peptide from the N-terminal to Met-110 in the cross-linked product was displaced from its position. Similar change in the mobility of the calmodulin peptides was also observed in the cross-linked products with troponin I. It was concluded, therefore, that at least one cross-linked site for myosin light chain kinase and one for troponin I were located between Met-73 and Met-110 of the wheat germ calmodulin.

The sites on calmodulin cross-linked to myosin light chain kinase and troponin I with water-soluble carbodiimide. Publishing Authors By Initials

K Yamamoto,T Sekine,K Sutoh,

For similar macromolecular substances: polymers: biopolymers: microfilament proteins: troponin: troponin i research abstracts see: macromolecular substances: polymers: biopolymers: microfilament proteins: troponin: troponin i research

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The sites on calmodulin cross-linked to myosin light chain kinase and troponin I with water-soluble carbodiimide. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 104

Page Numbers: 251-4

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1988

The sites on calmodulin cross-linked to myosin light chain kinase and troponin I with water-soluble carbodiimide. Information

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LANGUAGE: eng

NlmUniqueID: 376600

The sites on calmodulin cross-linked to myosin light chain kinase and troponin I with water-soluble carbodiimide. Keywords Mesh Terms:

KEYWORDS: Troponin I

MESH TERMS: metabolism

Chemical & Substance for Abstract: The sites on calmodulin cross-linked to myosin light chain kinase and troponin I with water-soluble carbodiimide. Information

Substance Name: Myosin-Light-Chain Kinase

Registry Number: EC 2.7.1.117

Grant and Affiliation Information for The sites on calmodulin cross-linked to myosin light chain kinase and troponin I with water-soluble carbodiimide.

AFFILIATION: Faculty of Liberal Arts, University of the Air, Chiba.

Country: JAPAN

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MEDLINETA: J Biochem

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